Summary Pseudomonas putida DOT‐T1E is a solvent‐tolerant strain able to grow in the
presence of> 1%(v/v) toluene in the culture medium. A set of multidrug efflux pumps have …

The size of the TOL plasmid pWW20 from Pseudomonas putida MT20, as measured by
analysis of agarose electrophoresis gels after restriction endonuclease hydrolysis, was 270 …

Plasmid pEST1463 carrying the promoterless pheBA operon was cloned into Pseudomonas
putida PaW85, and phenol-utilizing colonies were isolated on minimal plates containing …

Burkholderia (Pseudomonas) cepacia PR1 (23) has been shown to constitutively express to
toluene catabolic pathway distinguished by a unique toluene ortho-monooxygenase (Tom) …

The TOL plasmid upper pathway operon encodes enzymes involved in the catabolism of
aromatic hydrocarbons such as toluene and xylenes. The regulator of the gene pathway, the …

The functional expression of environmental genes in a particular host bacterium is
hampered by various limitations including inefficient transcription of target genes as well as …

The possibility of the accidental or deliberate release of genetically engineered
microorganisms into the environment has accentuated the need to study their survival in …

Hybrid plasmids containing the regulated meta-cleavage pathway operon of TOL plasmid
pWWO were mutagenized with transposon Tn1000 or Tn5. The resulting insertion mutant …

Plasmid pIG7 DNA cloned in Pseudomonas putida with the broad-host-range vectors
pRK290 and pKT240 expresses the genes encoding nephthalene oxidation in the presence …

Transcriptional lacZ fusions to the Pu and Pm promoters of the TOL (toluene degradation)
plasmid inserted in monocopy in the chromosome of Pseudomonas putida showed a very …