On-chip three-dimensional tumor spheroid formation and pump-less perfusion culture using gravity-driven cell aggregation and balanced droplet dispensing
This paper presents a spheroid chip in which three-dimensional (3D) tumor spheroids are
not only formed by gravity-driven cell aggregation but also cultured at the perfusion rates …
not only formed by gravity-driven cell aggregation but also cultured at the perfusion rates …
Mass fabrication of uniform sized 3D tumor spheroid using high-throughput microfluidic system
In vivo tumors develop in a three-dimensional manner and have unique and complex
characteristics. Physico-biochemical barriers on tumors cause drug resistance and limit drug …
characteristics. Physico-biochemical barriers on tumors cause drug resistance and limit drug …
A 3D printed microfluidic perfusion device for multicellular spheroid cultures
The advent of 3D printing technologies promises to make microfluidic organ-on-chip
technologies more accessible for the biological research community. To date, hydrogel …
technologies more accessible for the biological research community. To date, hydrogel …
Detachably assembled microfluidic device for perfusion culture and post‐culture analysis of a spheroid array
Y Sakai, K Hattori, F Yanagawa, S Sugiura… - Biotechnology …, 2014 - Wiley Online Library
Microfluidic devices permit perfusion culture of three‐dimensional (3D) tissue, mimicking the
flow of blood in vascularized 3D tissue in our body. Here, we report a microfluidic device …
flow of blood in vascularized 3D tissue in our body. Here, we report a microfluidic device …
[HTML][HTML] An inexpensive “do-it-yourself” device for rapid generation of uniform tumor spheroids
Summary Three-dimensional (3D) cancer cell culture models such as tumor spheroids better
recapitulate in vivo tumors than conventional two-dimensional models. However, two major …
recapitulate in vivo tumors than conventional two-dimensional models. However, two major …
Gradient-sized control of tumor spheroids on a single chip
Multicellular tumor spheroids are attracting more attention as a physiologically relevant in
vitro tumor model for biomedical research. The size of spheroids is one of the critical …
vitro tumor model for biomedical research. The size of spheroids is one of the critical …
A novel three-dimensional microfluidic platform for on chip multicellular tumor spheroid formation and culture
The formation of three-dimensional (3D) multicellular cell spheroids such as microspheres
and embryoid bodies has recently gained much attention as a useful cell culture technique …
and embryoid bodies has recently gained much attention as a useful cell culture technique …
Microfluidic self-assembly of tumor spheroids for anticancer drug discovery
LY Wu, D Di Carlo, LP Lee - Biomedical microdevices, 2008 - Springer
Creating multicellular tumor spheroids is critical for characterizing anticancer treatments
since it may provide a better model than monolayer culture of in vivo tumors. Moreover …
since it may provide a better model than monolayer culture of in vivo tumors. Moreover …
Uniform sized cancer spheroids production using hydrogel-based droplet microfluidics: a review
Abstract Three-dimensional (3D) cell culture models have been extensively utilized in
various mechanistic studies as well as for drug development studies as superior in vitro …
various mechanistic studies as well as for drug development studies as superior in vitro …
A multiscale, vertical-flow perfusion system with integrated porous microchambers for upgrading multicellular spheroid culture
M Takagi, M Yamada, R Utoh, M Seki - Lab on a Chip, 2023 - pubs.rsc.org
Spheroid formation assisted by microengineered chambers is a versatile approach for
morphology-controlled three-dimensional (3D) cell cultivation with physiological relevance …
morphology-controlled three-dimensional (3D) cell cultivation with physiological relevance …