Update on the epigenomic implication of embryo cryopreservation methods applied in assisted reproductive technologies with potential long-term health effects

A Reyes Palomares… - Frontiers in Cell and …, 2022 - frontiersin.org
Cryopreservation of embryos has become an efficient method in Assisted Reproductive
Technologies (ART) and these methods are currently performed at nearly all fertility centers …

Embryo survival and birth rate after minimum volume vitrification or slow freezing of in vivo and in vitro produced ovine embryos

PC Dos Santos-Neto, F Cuadro, N Barrera, M Crispo… - Cryobiology, 2017 - Elsevier
The objective was to evaluate pregnancy outcomes and birth rate of in vivo derived vs. in
vitro produced ovine embryos submitted to different cryopreservation methods. A total of 197 …

Transcriptomic difference in bovine blastocysts following vitrification and slow freezing at morula stage

A Gupta, J Singh, I Dufort, C Robert, FCF Dias… - PLoS …, 2017 - journals.plos.org
Cryopreservation is known for its marked deleterious effects on embryonic health. Bovine
compact morulae were vitrified or slow-frozen, and post-warm morulae were cultured to the …

Effect of vitrification using the Cryotop method on the gene expression profile of in vitro–produced bovine embryos

L de Oliveira Leme, I Dufort, JFW Spricigo, TF Braga… - Theriogenology, 2016 - Elsevier
The present study analyzed the changes in gene expression induced by the Cryotop
vitrification technique in bovine blastocyst-stage embryos, using Agilent EmbryoGENE …

Transfer of fresh and cryopreserved IVP bovine embryos: normal calving, birth weight and gestation lengths

Y Agca, RL Monson, DL Northey, OA Mazni… - Theriogenology, 1998 - Elsevier
In vitro and in vivo developmental competence of fresh and cryopreserved in vitro produced
(IVP) bovine embryos was evaluated up to birth. Three experiments were done. The …

Vitrification of in vitro produced ovine embryos at various developmental stages using two methods

A Shirazi, M Soleimani, M Karimi, H Nazari, E Ahmadi… - Cryobiology, 2010 - Elsevier
This study was conducted to evaluate the effects of developmental stage of in vitro produced
(IVP) ovine embryos and the type of vitrification procedure used on embryo cryotolerance …

Vitrification devices affect structural and molecular status of in vitro matured ovine oocytes

S Succu, GG Leoni, D Bebbere… - Molecular …, 2007 - Wiley Online Library
We evaluated the effect of three different cryodevices on membrane integrity, tubulin
polymerization, maturation promoting factor (MPF) activity and developmental competence …

Cryobanking of farm animal gametes and embryos as a means of conserving livestock genetics

L Mara, S Casu, A Carta, M Dattena - Animal Reproduction Science, 2013 - Elsevier
In the last few decades, farm animal genetic diversity has rapidly declined, mainly due to
changing market demands and intensification of agriculture. But, since the removal of single …

Current Status of In Vitro Embryo Production in Sheep and Goats

MT Paramio, D Izquierdo - Reproduction in Domestic Animals, 2014 - Wiley Online Library
Contents Sheep and goat production is an important economic activity in Spain with an
increasing interest in milk production. Multiovulation and Embryo Transfer (MOET) and In …

Consequences of In Vitro Culture Conditions on Embryo Development and Quality

D Rizos, M Clemente… - … in Domestic Animals, 2008 - Wiley Online Library
Contents Despite major efforts directed at improving the yield of blastocysts from immature
oocytes in vitro, the quality of such blastocysts continually lags behind that of blastocysts …