Goals and approaches for each processing step for single-cell RNA sequencing data
Single-cell RNA sequencing (scRNA-seq) has enabled researchers to study gene
expression at the cellular level. However, due to the extremely low levels of transcripts in a …
expression at the cellular level. However, due to the extremely low levels of transcripts in a …
Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows
you to profile the whole transcriptome of a large number of individual cells. However, the …
you to profile the whole transcriptome of a large number of individual cells. However, the …
[HTML][HTML] Single-cell transcriptomics bioinformatics and computational challenges
The emerging single-cell RNA-Seq (scRNA-Seq) technology holds the promise to
revolutionize our understanding of diseases and associated biological processes at an …
revolutionize our understanding of diseases and associated biological processes at an …
[HTML][HTML] Data analysis guidelines for single-cell RNA-seq in biomedical studies and clinical applications
M Su, T Pan, QZ Chen, WW Zhou, Y Gong, G Xu… - Military Medical …, 2022 - Springer
The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has
advanced our understanding of the pathogenesis of disease and provided valuable insights …
advanced our understanding of the pathogenesis of disease and provided valuable insights …
The triumphs and limitations of computational methods for scRNA-seq
PV Kharchenko - Nature methods, 2021 - nature.com
The rapid progress of protocols for sequencing single-cell transcriptomes over the past
decade has been accompanied by equally impressive advances in the computational …
decade has been accompanied by equally impressive advances in the computational …
[HTML][HTML] A systematic evaluation of single cell RNA-seq analysis pipelines
The recent rapid spread of single cell RNA sequencing (scRNA-seq) methods has created a
large variety of experimental and computational pipelines for which best practices have not …
large variety of experimental and computational pipelines for which best practices have not …
[HTML][HTML] Comparative analysis of sequencing technologies for single-cell transcriptomics
Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we
present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq …
present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq …
[HTML][HTML] Design and computational analysis of single-cell RNA-sequencing experiments
R Bacher, C Kendziorski - Genome biology, 2016 - Springer
Single-cell RNA-sequencing (scRNA-seq) has emerged as a revolutionary tool that allows
us to address scientific questions that eluded examination just a few years ago. With the …
us to address scientific questions that eluded examination just a few years ago. With the …
[HTML][HTML] Single-cell RNA sequencing and its combination with protein and DNA analyses
JR Choi, KW Yong, JY Choi, AC Cowie - Cells, 2020 - mdpi.com
Heterogeneity in cell populations poses a significant challenge for understanding complex
cell biological processes. The analysis of cells at the single-cell level, especially single-cell …
cell biological processes. The analysis of cells at the single-cell level, especially single-cell …
[HTML][HTML] Comparative analysis of single-cell RNA sequencing methods
Single-cell RNA sequencing (scRNA-seq) offers new possibilities to address biological and
medical questions. However, systematic comparisons of the performance of diverse scRNA …
medical questions. However, systematic comparisons of the performance of diverse scRNA …