Coactivation in vitro of the sigma54-dependent promoter Pu of the TOL plasmid of Pseudomonas putida by HU and the mammalian HMG-1 protein

J Pérez-Martín, V De Lorenzo - Journal of bacteriology, 1997 - Am Soc Microbiol
The mechanism by which the prokaryotic histone-like protein HU replaces the integration
host factor (IHF) in the coactivation of the sigma54-dependent promoter Pu of Pseudomonas …

The sigma 54-dependent promoter Ps of the TOL plasmid of Pseudomonas putida requires HU for transcriptional activation in vivo by XylR

J Pérez-Martín, V de Lorenzo - Journal of bacteriology, 1995 - Am Soc Microbiol
In the presence of toluene and xylenes, the sigma 54-dependent Ps promoter of the TOL
(toluene biodegradation) plasmid pWW0 of Pseudomonas putida is activated at a distance …

Integration host factor suppresses promiscuous activation of the sigma 54-dependent promoter Pu of Pseudomonas putida.

J Pérez-Martín, V De Lorenzo - Proceedings of the National …, 1995 - National Acad Sciences
In the presence of m-xylene, the Pu promoter of the TOL plasmid of Pseudomonas putida is
activated by the prokaryotic enhancer-binding protein XylR. The intervening DNA segment …

Involvement of σ54 in exponential silencing of the Pseudomonas putida TOL plasmid Pu promoter

II Cases, V de Lorenzo… - Molecular microbiology, 1996 - Wiley Online Library
The σ54‐dependent Pu promoter of the TOL plasmid pWWO of Pseudomonas putida
becomes activated by the prokaryotic enhancer‐binding XyIR protein when cells encounter …

Co-regulation by bent DNA. Functional substitutions of the integration host factor site at sigma 54-dependent promoter Pu of the upper-TOL operon by intrinsically …

J Perez-Martin, KN Timmis, V De Lorenzo - Journal of Biological Chemistry, 1994 - Elsevier
The role of integration host factor (IHF) in the regulation of the sigma 54-dependent promoter
Pu of the TOL plasmid of Pseudomonas putida has been examined. We have selected in …

In Vivo UV Laser Footprinting of thePseudomonas putida ς54PuPromoter Reveals That Integration Host Factor Couples Transcriptional Activity to Growth Phase

M Valls, M Buckle, V de Lorenzo - Journal of Biological Chemistry, 2002 - ASBMB
The occupation of the ς 54-dependent Pu promoter of Pseudomonas putida by the
integration host factor (IHF) under different growth conditions has been monitored in its …

Transient XylR binding to the UAS of the Pseudomonas putida σ54 promoter Pu revealed with high intensity UV footprinting in vivo

M Valls, V de Lorenzo - Nucleic acids research, 2003 - academic.oup.com
The binding of the transcriptional regulator XylR to its cognate upstream activating
sequences (UAS) of the σ54‐dependent promoter Pu of Pseudomonas putida has been …

Upstream binding sequences of the XyIR activator protein and integration host factor in the xylS gene promoter region of the Pseudomonas TOL plasmid

A Holtel, KN Timmis, JL Ramos - Nucleic acids research, 1992 - academic.oup.com
The xylR and xylS genes, which encode the positive regulators of the TOL plasmid catabolic
pathways, are adjacent genes on the TOL plasmid and are transcribed from divergent …

Recruitment of RNA Polymerase Is a Rate-limiting Step for the Activation of the ς54 Promoter Pu ofPseudomonas putida

M Carmona, V de Lorenzo, G Bertoni - Journal of Biological Chemistry, 1999 - ASBMB
The activity of the ς 54-promoter Pu of Pseudomonas putida was examined in vitro with a
DNA template lacking upstream activating sequences, such that RNA polymerase can be …

Functional analysis of the integration host factor site of the σ54Pu promoter of Pseudomonas putida by in vivo UV imprinting

M Valls, R Silva‐Rocha, I Cases, A Muñoz… - Molecular …, 2011 - Wiley Online Library
The integration host factor (IHF) of Pseudomonas putida connects cell growth to
transcriptional activity of distinct promoters. The IHF site of the σ54 promoter Pu of the TOL …