A major bottleneck for large‐scale and volume EM is the imaging speed. The total
acquisition time needed for a single sample can easily take days, or even weeks using …

In a few successive PhD projects, we transformed a FEI Nova-nano SEM column into an
instrument with an array of 14x14 focused electron beams on the sample [1-3]. The original …

Our group is developing a multibeam scanning electron microscope (SEM) with 196 beams
in order to increase the throughput of SEM. Three imaging systems using, respectively …

Conventional scanning electron microscopes are limited in their ultimate data acquisition
rate at a given resolution by statistical electron-electron interaction (so-called Coulomb …

We have developed a multi-beam scanning electron microscope (MBSEM), which delivers a
square array of 196 focused beams onto a sample with a resolution and current per beam …

Large-scale and volume electron microscopy (EM) has revolutionized the understanding of
biological ultrastructure across different spatial scales, but the low throughput of EM …

Electron microscopy (EM) is increasingly being used in large-scale biological projects, either
for volume imaging or large area mapping. In both use cases, a need for increased …

Large area electron microscopy (EM) imaging has long been difficult due to fundamental
limits in throughput for conventional electron microscopes. New developments in …

To achieve faster imaging in scanning electron microscopy, novel imaging methods are
needed, such as multi-beam scanning electron microscopes [1, 2]. Our scanning electron …

Miniaturization of electron optical systems has gained much interest over the last decade [1,
2]. In a scanning electron microscope, downscaling of the column dimensions is expected to …