[HTML][HTML] Classification of antimicrobial mechanism of action using dynamic bacterial morphology imaging

X Ouyang, J Hoeksma, RJM Lubbers, TK Siersma… - Scientific Reports, 2022 - nature.com
Antimicrobial resistance is a major threat to human health. Basic knowledge of antimicrobial
mechanism of action (MoA) is imperative for patient care and for identification of novel …

From single bacterial cell imaging towards in vivo single-molecule biochemistry studies

U Endesfelder - Essays in Biochemistry, 2019 - portlandpress.com
Bacteria as single-cell organisms are important model systems to study cellular mechanisms
and functions. In recent years and with the help of advanced fluorescence microscopy …

High‐throughput dilution‐based growth method enables time‐resolved exo‐metabolomics of Pseudomonas putida and Pseudomonas aeruginosa

BH Pedersen, N Gurdo, HK Johansen… - Microbial …, 2021 - Wiley Online Library
Understanding metabolism is fundamental to access and harness bacterial physiology. In
most bacteria, nutrient utilization is hierarchically optimized according to their energetic …

Fast single-cell biochemistry: theory, open source microscopy and applications

AL Trinh, S Ber, A Howitt, PO Valls… - … and applications in …, 2019 - iopscience.iop.org
Fluorescence lifetime sensing enables researchers to probe the physicochemical
environment of a fluorophore providing a window through which we can observe the …

Live-cell fluorescence microscopy to investigate subcellular protein localization and cell morphology changes in bacteria

RS Brzozowski, ML White, PJ Eswara - JoVE (Journal of Visualized …, 2019 - jove.com
Investigations of factors influencing cell division and cell shape in bacteria are commonly
performed in conjunction with high-resolution fluorescence microscopy as observations …

Pulses and delays, anticipation and memory: seeing bacterial stress responses from a single-cell perspective

V Lagage, S Uphoff - FEMS microbiology reviews, 2020 - academic.oup.com
Stress responses are crucial for bacteria to survive harmful conditions that they encounter in
the environment. Although gene regulatory mechanisms underlying stress responses in …

Mid-Infrared Photothermal–Fluorescence In Situ Hybridization for Functional Analysis and Genetic Identification of Single Cells

Y Bai, Z Guo, FC Pereira, M Wagner… - Analytical …, 2023 - ACS Publications
Simultaneous identification and metabolic analysis of microbes with single-cell resolution
and high throughput are necessary to answer the question of “who eats what, when, and …

Design of a line-of-sight fluorescence-based imaging diagnostic for classification of microbe species

JM Herzog, V Sick - Measurement Science and Technology, 2023 - iopscience.iop.org
Fluorescence imaging of certain biochemicals, including flavins and pyridine nucleotides,
has utility in characterizing the metabolic state of tissue and in discriminating between …

[HTML][HTML] Evaluation and characterization of bacterial metabolic dynamics with a novel profiling technique, real-time metabolotyping

S Fukuda, Y Nakanishi, E Chikayama, H Ohno… - PLoS …, 2009 - journals.plos.org
Background Environmental processes in ecosystems are dynamically altered by several
metabolic responses in microorganisms, including intracellular sensing and pumping, battle …

High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy

AJ Bower, J Li, EJ Chaney, M Marjanovic, DR Spillman… - Optica, 2018 - opg.optica.org
Two-photon fluorescence lifetime imaging microscopy (2P-FLIM) of autofluorescent
metabolic coenzymes has been widely used to investigate energetic perturbations in living …