Live-cell fluorescence microscopy to investigate subcellular protein localization and cell morphology changes in bacteria
Investigations of factors influencing cell division and cell shape in bacteria are commonly
performed in conjunction with high-resolution fluorescence microscopy as observations …
performed in conjunction with high-resolution fluorescence microscopy as observations …
High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy
Two-photon fluorescence lifetime imaging microscopy (2P-FLIM) of autofluorescent
metabolic coenzymes has been widely used to investigate energetic perturbations in living …
metabolic coenzymes has been widely used to investigate energetic perturbations in living …
Design of a line-of-sight fluorescence-based imaging diagnostic for classification of microbe species
Fluorescence imaging of certain biochemicals, including flavins and pyridine nucleotides,
has utility in characterizing the metabolic state of tissue and in discriminating between …
has utility in characterizing the metabolic state of tissue and in discriminating between …
[HTML][HTML] Evaluation and characterization of bacterial metabolic dynamics with a novel profiling technique, real-time metabolotyping
S Fukuda, Y Nakanishi, E Chikayama, H Ohno… - PLoS …, 2009 - journals.plos.org
Background Environmental processes in ecosystems are dynamically altered by several
metabolic responses in microorganisms, including intracellular sensing and pumping, battle …
metabolic responses in microorganisms, including intracellular sensing and pumping, battle …
Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling
Label-free molecular imaging techniques such as matrix-assisted laser desorption ionization
mass spectrometry imaging (MALDI-MSI) enable the direct and simultaneous mapping of …
mass spectrometry imaging (MALDI-MSI) enable the direct and simultaneous mapping of …
In vivo imaging and tracking of host–microbiota interactions via metabolic labeling of gut anaerobic bacteria
N Geva-Zatorsky, D Alvarez, JE Hudak, NC Reading… - Nature medicine, 2015 - nature.com
The intestine is densely populated by anaerobic commensal bacteria. These
microorganisms shape immune system development, but understanding of host–commensal …
microorganisms shape immune system development, but understanding of host–commensal …
Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice
Real-time localization and microbial activity information of indigenous gut microbiota over an
extended period of time remains a challenge with existing visualizing methods. Here, we …
extended period of time remains a challenge with existing visualizing methods. Here, we …
Review of methods to probe single cell metabolism and bioenergetics
AE Vasdekis, G Stephanopoulos - Metabolic engineering, 2015 - Elsevier
Single cell investigations have enabled unexpected discoveries, such as the existence of
biological noise and phenotypic switching in infection, metabolism and treatment. Herein …
biological noise and phenotypic switching in infection, metabolism and treatment. Herein …
Real-time chemical imaging of bacterial activity in biofilms using open-channel microfluidics and synchrotron FTIR spectromicroscopy
Real-time chemical imaging of bacterial activities can facilitate a comprehensive
understanding of the dynamics of biofilm structures and functions. Synchrotron-radiation …
understanding of the dynamics of biofilm structures and functions. Synchrotron-radiation …
High‐throughput quantification of microbial birth and death dynamics using fluorescence microscopy
Background Microbes live in dynamic environments where nutrient concentrations fluctuate.
Quantifying fitness in terms of birth rate and death rate in a wide range of environments is …
Quantifying fitness in terms of birth rate and death rate in a wide range of environments is …