Genetically modified organisms (GMOs) are commonly used to produce valuable
compounds in closed industrial systems. However, their emerging applications in open …

Genetically modified organisms (GMOs) are increasingly deployed at large scales and in
open environments. Genetic biocontainment strategies are needed to prevent unintended …

In the process of clone-based genome sequencing, initial assemblies frequently contain
cloning gaps that can be resolved using cloning-independent methods, but the reason for …

As the field of synthetic biology develops, real-world applications are moving from the
realms of ideas and laboratory-confined research towards implementation. A pressing …

The increasing use of engineered organisms for industrial, clinical, and environmental
applications poses a growing risk of spreading hazardous biological entities into the …

Genetically modified organisms (GMOs) are increasingly used in research and industrial
systems to produce high-value pharmaceuticals, fuels and chemicals. Genetic isolation and …

As the use of synthetic biology both in industry and in academia grows, there is an
increasing need to ensure biocontainment. There is growing interest in engineering …

Synthetic biology has promised and delivered on an impressive array of applications based
on genetically modified microorganisms. While novel biotechnology undoubtedly offers …

Highlights•The quest for minimal genomes has both fundamental and biotechnological
motivations.•Genome reduction has been enabled by chromosomal editing and chemical …

Biocontainment systems that couple environmental sensing with circuit-based control of cell
viability could be used to prevent escape of genetically modified microbes into the …