Human plasma is regarded the most complex and well-known clinical specimen that can be
easily obtained; alterations in the levels of plasma proteins or their corresponding enzyme …

The rapid development in proteomics over the last 10 years has led to a series of new
technologies and combinations of them designed to unravel as much as possible of the …

Numerous recent reports (eg Refs. 1–3) suggest that proteome studies are in the process of
finding a range of novel disease biomarkers in plasma and serum, giving rise to the hope …

This article provides a comprehensive updated review of three proteomics technologies (2D
gel electrophoresis [2DE]; chromatin immunoprecipitation [ChIP] and its related alternates …

Here, approaches that allow disclosure of the information hidden inside and outside of two-
dimensional gel electrophoresis (2DE) are described. Experimental identification methods …

Two key technologies in experimental proteomics are two-dimensional polyacrylamide gel
electrophoresis (2D PAGE) and electrospray or laser desorption mass spectrometry. The …

Since the turn of the century, mass spectrometry (MS) technologies have continued to
improve dramatically, and advanced strategies that were impossible a decade ago are …

Summary Two-dimensional (2-D) gel electrophoresis concerted with protein identification by
mass spectrometry (MS) is an extremely powerful method for comparative expression …

We present some current definitions related to functional and structural proteomics and the
human proteome, and we review the following aspects of proteome analysis: Classical 2-D …

Proteomic approaches to the identification of novel biomarkers for cancer diagnosis and
staging have traditionally relied on the identification of differentially expressed proteins …