Golden Standard: a complete standard, portable, and interoperative MoClo tool for model and non-model proteobacteria

B Blázquez, DS León, J Torres-Bacete… - Nucleic Acids …, 2023 - academic.oup.com
Modular cloning has become a benchmark technology in synthetic biology. However, a
notable disparity exists between its remarkable development and the need for …

EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology

SJ Moore, HE Lai, RJR Kelwick, SM Chee… - ACS Synthetic …, 2016 - ACS Publications
Golden Gate cloning is a prominent DNA assembly tool in synthetic biology for the assembly
of plasmid constructs often used in combinatorial pathway optimization, with a number of …

CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology

SV Iverson, TL Haddock, J Beal… - ACS synthetic …, 2016 - ACS Publications
Multipart and modular DNA part libraries and assembly standards have become common
tools in synthetic biology since the publication of the Gibson and Golden Gate assembly …

[HTML][HTML] Discovering the next decade's synthetic biology research trends with ChatGPT

Y Tong, L Zhang - Synthetic and Systems Biotechnology, 2023 - ncbi.nlm.nih.gov
ChatGPT, a conversational AI (Artificial Intelligence) model developed and recently released
by OpenAI (https://openai. com/), caused quite a stir. However, the debate of AI never fades …

Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo system

S Werner, C Engler, E Weber, R Gruetzner… - …, 2012 - Taylor & Francis
Recent progress in the field of synthetic biology has led to the creation of cells containing
synthetic genomes. Although these first synthetic organisms contained copies of natural …

SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities

E Martínez-García, T Aparicio… - Nucleic acids …, 2015 - academic.oup.com
Abstract The Standard European Vector Architecture 2.0 database (SEVA-DB 2.0,
http://seva. cnb. csic. es) is an improved and expanded version of the platform released in …

RoboMoClo: a robotics-assisted modular cloning framework for multiple gene assembly in biofoundry

DH Kang, SC Ko, YB Heo, HJ Lee… - ACS Synthetic …, 2022 - ACS Publications
Efficient and versatile DNA assembly frameworks have had an impact on promoting
synthetic biology to build complex biological systems. To accelerate system development …

Synthetic biology: Learning the way toward high-precision biological design

S Kitano, C Lin, JL Foo, MW Chang - PLoS Biology, 2023 - journals.plos.org
Synthetic biology: Learning the way toward high-precision biological design | PLOS Biology
Skip to main content Advertisement PLOS Biology Browse Current Issue Journal Archive …

Overloaded and stressed: whole-cell considerations for bacterial synthetic biology

O Borkowski, F Ceroni, GB Stan, T Ellis - Current opinion in microbiology, 2016 - Elsevier
Highlights•Predictability and robustness are major challenges for synthetic
biology.•Engineering bacteria requires putting synthetic constructs into growing host …

[HTML][HTML] A navigation guide of synthetic biology tools for Pseudomonas putida

M Martin-Pascual, C Batianis, L Bruinsma… - Biotechnology …, 2021 - Elsevier
Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …