Size exclusion chromatography–multiangle laser light scattering analysis of hyaluronan size distributions made by membrane-bound hyaluronan synthase
BA Baggenstoss, PH Weigel - Analytical Biochemistry, 2006 - Elsevier
Size exclusion chromatography–multiangle laser light scattering (SEC–MALLS) analyses of
Escherichia coli membranes expressing Streptococcus equisimilis hyaluronan synthase …
Escherichia coli membranes expressing Streptococcus equisimilis hyaluronan synthase …
Chemical, enzymatic and biological synthesis of hyaluronic acids
Hyaluronic acid (HA) is a major glycosaminoglycan, a family of structurally complex, linear,
anionic hetero-co-polysaccharides. HA is important in various anatomical structures …
anionic hetero-co-polysaccharides. HA is important in various anatomical structures …
Comprehensive review on biosynthesis of hyaluronic acid with different molecular weights and its biomedical applications
F Jabbari, V Babaeipour, S Saharkhiz - International Journal of Biological …, 2023 - Elsevier
Hyaluronic acid (HA), an anionic and nonsulfated glycosaminoglycan, is the main structural
component of various tissues and plays an important role in various biological processes …
component of various tissues and plays an important role in various biological processes …
Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties
N Itano, T Sawai, M Yoshida, P Lenas… - Journal of Biological …, 1999 - ASBMB
Three mammalian hyaluronan synthase genes, HAS1, HAS2, and HAS3, have recently
been cloned. In this study, we characterized and compared the enzymatic properties of …
been cloned. In this study, we characterized and compared the enzymatic properties of …
Hyaluronan synthase control of synthesis rate and hyaluronan product size are independent functions differentially affected by mutations in a conserved tandem BX7 …
BA Baggenstoss, EN Harris, JL Washburn… - …, 2017 - academic.oup.com
Hyaluronan synthases (HAS) normally make large (> MDa) hyaluronan (HA) products.
Smaller HA fragments (eg 100–400 kDa) produced in vivo are associated with inflammation …
Smaller HA fragments (eg 100–400 kDa) produced in vivo are associated with inflammation …
Enzyme variants in biosynthesis and biological assessment of different molecular weight hyaluronan
T Ebrahimi, M Keramati, F Khodabakhsh, RA Cohan - AMB Express, 2024 - Springer
In the present study, low-and high-molecular-weight hyaluronic acids (LMW-HA and HMW-
HA) were synthesized in vitro by truncated Streptococcus equisimilis hyaluronan synthases …
HA) were synthesized in vitro by truncated Streptococcus equisimilis hyaluronan synthases …
Alteration of polysaccharide size distribution of a vertebrate hyaluronan synthase by mutation
PE Pummill, PL DeAngelis - Journal of Biological Chemistry, 2003 - ASBMB
Hyaluronan (HA) is a nonsulfated glycosaminoglycan that has long been known to play
structural roles in vertebrates. Recently, it has become increasingly obvious that this linear …
structural roles in vertebrates. Recently, it has become increasingly obvious that this linear …
Mutation of two intramembrane polar residues conserved within the hyaluronan synthase family alters hyaluronan product size
K Kumari, BA Baggenstoss, AL Parker… - Journal of Biological …, 2006 - ASBMB
We identified two conserved polar amino acids within different membrane domains (MD) of
Streptococcus equisimilis hyaluronan synthase (seHAS), Lys 48 in MD2 and Glu 327 in …
Streptococcus equisimilis hyaluronan synthase (seHAS), Lys 48 in MD2 and Glu 327 in …
Hyaluronan synthases
PH Weigel, VC Hascall, M Tammi - Journal of Biological Chemistry, 1997 - ASBMB
In 1934, Meyer and Palmer isolated a novel, high Mr glycosaminoglycan from the vitreous of
the eye (1). They showed that this substance contained a hexuronic acid, an amino sugar …
the eye (1). They showed that this substance contained a hexuronic acid, an amino sugar …