A secondary, spectrophotometric assay using p-nitrophenyl acetate as substrate has been
developed for the determination of the specific activity of chicken liver carboxylesterase. A …

Identical kcat values (≈ 40 s− 1) are obtained for the chicken liver carboxylesterase
catalyzed hydrolysis of phenyl, p-nitrophenyl and o-nitrophenyl benzoates providing support …

Publisher Summary This chapter discusses the determination of carboxylesterases from
chicken, sheep, and horse liver. In the course of purification procedure for chicken liver …

In 1930, Bamann and Schmeller1-2clearly showed that ethyl phenylglyoxylate is a much
poorer substrate than ethyl (±)-mandelate for various liver carboxylesterases, 3 and …

Chicken and ox liver carboxylesterases are inhibited irreversibly by α-bromoacetophenone.
A kinetic analysis of the process indicates that inhibition occurs at the active site by reaction …

Carboxylesterase ESB3 was extracted from ovine liver and purified to homogeneity by
ammonium sulphate fractionation, hydrophobic interaction chromatography on Phenyl …

Carboxylesterase was purified from camel liver through ammonium sulphate precipitation,
chromatography on DEAE-Sepharose and gel filtration on a Sepharose 6B. The molecular …

The reactions of 3, 3, 3--trifluoro-2, 2-dihydroxy-l-phenyl-l-propanone (TDPP [with chicken
liver carboxylesterase have shown that this ketone hydrate is not only a potent inhibitor of …

Publisher Summary This chapter discusses the determination of carboxylesterases from pig
and ox liver. Carboxylesterases are remarkably efficient enzymes that catalyze the …

We have recently synthesized a series of phosphorylated flavonoids and identified some of
them as potent inhibitors of pancreatic cholesterol esterase (CEase) with excellent selectivity …