Although some information is available on the nutrient requirements of preimplantation
stages of mammalian embryos, much more work is required before we have a …

The last ten years have seen major advances in the culture of mammalian embryos during
the pre-implantation period. Mouse oocytes have undergone maturation in vitro, followed by …

Abstract 407 fertilized rabbit ova and 464 mouse ova were obtained from superovulated
donors 24-96 hrs. after mating. Krebs-Ringer bicarbonate, Eagle's basal medium, rabbit …

Early culture systems for sustaining the development of embryos from domesticated animals,
eg, bovine, ovine and porcine, were of two kinds that were designed to meet different …

Explantation methods have shown inconstant results when applied to the problems of
mammalian development. The results obtained with rabbit material have so far been …

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Simple and reliable methods are now available for growing rat and mouse embryos in
culture at all stages of organogenesis. Primitive‐streak embryos can be maintained for up to …

All normal mouse embryos develop from the pronuclear stage to blastocysts when cultured
in a simple chemically defined medium and a high proportion of these develop into normal …

The search for an effective culture medium for postimplantation mammalian embryos is
considered from two viewpoints: identification of the medium that gives the best …

Scientific investigations during the last three decades have led to the present capability of
nurturing the embryonic development from zygote to blastocyst stages in vitro for several …