In comparison to mouse, the developmental process of human islets has not been properly
elucidated. The advancement of single cell RNA-seq technology enables us to study the …

Investigating pancreatic islet differentiation from human stem cells in vitro provides a unique
opportunity to dissect mechanisms that operate during human development in utero. We …

Objective Dedifferentiation of pancreatic β-cells may reduce islet function in type 2 diabetes
(T2D). However, the prevalence, plasticity and functional consequences of this cellular state …

In this issue of Developmental Cell, Zhu et al. provide integrated data from single-cell/nuclei
RNAseq and chromatin accessibility (ATAC) across both a multistaged differentiation …

The in vitro differentiation of insulin-producing beta-like cells can model aspects of human
pancreatic development. Here, we generate 95,308 single-cell transcriptomes and …

Type 1 diabetes (T1D) is an autoimmune disease characterized by the destruction of beta
cells by immune cells. The interactions among cells within the islets may be closely linked to …

Current differentiation protocols have not been successful in reproducibly generating fully
functional human beta cells in vitro, partly due to incomplete understanding of human …

Prior studies have shown that pancreatic α-cells can transdifferentiate into β-cells, and that β-
cells de-differentiate and are prone to acquire an α-cell phenotype in type 2 diabetes (T2D) …

The pancreatic islet contains multiple hormone+ endocrine lineages (α, β, δ, PP and ε cells),
but the developmental processes that underlie endocrinogenesis are poorly understood …

Stem‐cell‐derived tissues offer platforms to study organ development, model physiology
during health and disease, and test novel therapies. We describe methods to isolate cells at …