Sequences capable of restoring poly (A) site function define two distinct downstream elements.
MA McDevitt, RP Hart, WW Wong, JR Nevins - The EMBO Journal, 1986 - embopress.org
Several recent studies have shown that a functional poly (A) site consists of both an
AAUAAA element as well as sequences downstream of the cleavage site. Two downstream …
AAUAAA element as well as sequences downstream of the cleavage site. Two downstream …
Multiple factors are required for specific RNA cleavage at a poly (A) addition site.
GM Gilmartin, MA McDevitt, JR Nevins - Genes & development, 1988 - genesdev.cshlp.org
An SP6 RNA containing the adenovirus 5 L3 poly (A) site is processed efficiently in a HeLa
cell nuclear extract to generate correct 3'termini. Accurate 3'processing has also been …
cell nuclear extract to generate correct 3'termini. Accurate 3'processing has also been …
Poly (A) site efficiency reflects the stability of complex formation involving the downstream element.
EA Weiss, GM Gilmartin, JR Nevins - The EMBO Journal, 1991 - embopress.org
A critical step in mRNA biogenesis is the generation of the mRNA 3′ end through an
endonucleolytic cleavage of the primary transcript followed by the addition of a …
endonucleolytic cleavage of the primary transcript followed by the addition of a …
Definition of essential sequences and functional equivalence of elements downstream of the adenovirus E2A and the early simian virus 40 polyadenylation sites
RP Hart, MA McDevitt, H Ali… - Molecular and cellular …, 1985 - Taylor & Francis
In addition to the highly conserved AATAAA sequence, there is a requirement for specific
sequences downstream of polyadenylic acid [poly (A)] cleavage sites to generate correct …
sequences downstream of polyadenylic acid [poly (A)] cleavage sites to generate correct …
Poly (A) site cleavage in a HeLa nuclear extract is dependent on downstream sequences
RP Hart, MA McDevitt, JR Nevins - Cell, 1985 - cell.com
Efficient utilization of the early SV40 poly (A) site in vivo requires sequences between 5 bp
and 18 bp downstream of the cleavage site. We have used a HeLa nuclear extract to …
and 18 bp downstream of the cleavage site. We have used a HeLa nuclear extract to …
Alternative poly (A) site utilization during adenovirus infection coincides with a decrease in the activity of a poly (A) site processing factor
KP Mann, EA Weiss, JR Nevins - Molecular and cellular biology, 1993 - Taylor & Francis
The recognition and processing of a pre-mRNA to create a poly (A) addition site, a
necessary step in mRNA biogenesis, can also be a regulatory event in instances in which …
necessary step in mRNA biogenesis, can also be a regulatory event in instances in which …
Two phases in the addition of a poly (A) tail.
MD Sheets, M Wickens - Genes & development, 1989 - genesdev.cshlp.org
The addition of a poly (A) tail has been examined in a HeLa cell nuclear extract using SV40
late RNAs that end at or near the natural poly (A) site. We find that the addition of a full …
late RNAs that end at or near the natural poly (A) site. We find that the addition of a full …
RNA structure is a critical determinant of poly (A) site recognition by cleavage and polyadenylation specificity factor
BR Graveley, ES Fleming… - Molecular and Cellular …, 1996 - Taylor & Francis
Sequence conservation among mammalian poly (A) sites is limited to the sequence
AAUAAA, coupled with an amorphous downstream U-or GU-rich region. Since these …
AAUAAA, coupled with an amorphous downstream U-or GU-rich region. Since these …
[引用][C] Poly (A) signals
N Proudfoot - Cell, 1991 - Elsevier
Most eukaryotic mRNAs have a poly (A) tail added onto their 3'ends, probably as an early
step in the maturation of the primary transcript. This process involves both cleavage of the …
step in the maturation of the primary transcript. This process involves both cleavage of the …
Assembly of the cleavage and polyadenylation apparatus requires about 10 seconds in vivo and is faster for strong than for weak poly (A) sites
LC Chao, A Jamil, SJ Kim, L Huang… - Molecular and cellular …, 1999 - Taylor & Francis
We have devised a cis-antisense rescue assay of cleavage and polyadenylation to
determine how long it takes the simian virus 40 (SV40) early poly (A) signal to commit itself …
determine how long it takes the simian virus 40 (SV40) early poly (A) signal to commit itself …