THE polysomal mRNA of eukaryotes is associated with at least two tightly binding proteins of
approximate molecular weights 75,000 and 60, 0001–5. The 75,000 MW polypeptide (P75) …

Several polyadenylylation (PA) signals containing elements upstream of the AAUAAA have
recently been characterized. Similar to PA elements found downstream of the AAUAAA, the …

We have partially purified a poly (A) polymerase (PAP) from HeLa cell nuclear extract which
is involved in the 3′-end formation of polyadenylated mRNA. PAP had a molecular weight …

To study the mechanism and factors required to form the 3'ends of polyadenylated mRNAs,
we have fractionated HeLa cell nuclear extracts carrying out the normally coupled cleavage …

Certain maternal mRNAs lose their poly (A) tails during early development and
concomitantly become translationally inactive. In this report we analyze the mechanism of …

The 3'terminus of simian virus 40 late mRNA is formed by nucleolytic cleavage of an mRNA
precursor. In this report we show that efficient cleavage requires a sequence in the 3'flanking …

Cleavage and polyadenylation factor (CPF) is required for the cleavage as well as for the
subsequent polyadenylation reaction during 3′ processing of messenger RNA precursors …

Polyadenylation of premessenger RNAs occurs posttranscriptionally in the nucleus of
eukaryotic cells by cleavage of the precursor and polymerization of adenosine residues. In …

Our previous studies of the 3′-end processing of simian virus 40 late mRNAs indicated the
existence of an essential element (or elements) downstream of the AAUAAA signal. We …

Publisher Summary This chapter discusses the regulatory function of Poly (A) and adjacent
3'sequences in translated ribonucleic acids (RNAs). Messenger RNA chains from various …