Bacteria expand their genetic diversity, spread antibiotic resistance genes, and obtain
virulence factors through the highly coordinated process of conjugative plasmid transfer …

Relaxosomes are specific nucleoprotein structures involved in DNA‐processing reactions
during bacterial conjugation. In this work, we present evidence indicating that plasmid R388 …

F plasmid‐mediated bacterial conjugation requires interactions between a relaxosome
component, TraM, and the coupling protein TraD, a hexameric ring ATPase that forms the …

Antibiotic resistance is a serious global problem. Antibiotic resistance genes (ARG), which
are widespread in environmental bacteria, can be transferred to pathogenic bacteria via …

Bacterial type IV secretion systems (T4SS) mediate interbacterial conjugative DNA transfer
and transkingdom protein transfer into eukaryotic host cells in bacterial pathogenesis. The …

Type IV secretion systems (T4SSs) are bacterial multiprotein organelles specialised in the
transfer of (nucleo) protein complexes across cell membranes. They are essential for …

We isolated and characterized traD mutants with an altered specificity of interaction with
relaxosomes of various conjugative (F and R388) and mobilizable (RSF1010 and ColE1) …

The type IV secretion system (T4SS) VirB/D4 of the facultative intracellular pathogen
Bartonella henselae is known to translocate bacterial effector proteins into human cells. Two …

TrwB is an integral membrane protein linking the relaxosome to the DNA transport
apparatus in plasmid R388 conjugation. Native TrwB has been purified in monomeric and …

The tra operon of the prototypical F plasmid and its relatives enables transfer of a copy of the
plasmid to other bacterial cells via the process of conjugation. Tra proteins assemble to form …