Cell-free expression offers an interesting alternative method to produce membrane proteins
in high amounts. Elimination of toxicity problems, reduced proteolytic degradation and a …

Cell-free expression has emerged as a promising tool for the fast and efficient production of
membrane proteins. The rapidly growing number of successfully produced targets in …

Cell-free expression techniques have emerged as promising tools for the production of
membrane proteins for structural and functional analysis. Elimination of toxic effects and a …

Cell-free protein synthesis is advantageous for membrane protein production because of the
potential lethality of membrane proteins synthesized in cell-based systems. Additionally the …

Different overexpression systems are widely used in the laboratory to produce proteins in a
reasonable amount for functional and structural studies. However, to optimize these systems …

Cell-free expression has emerged as a powerful technique to overcome major restrictions of
classical in vivo membrane protein production, with sample yields of mgms of protein per ml …

Cell-free protein production has become a core technology in the rapidly spreading field of
synthetic biology. In particular the synthesis of membrane proteins, highly problematic …

Membrane proteins are highly underrepresented in structural data banks due to tremendous
difficulties that occur upon approaching their structural analysis. Inefficient sample …

Publisher Summary Membrane proteins have become an important focus of the current
efforts in structural and functional genomics and the rapid progress of various genome …

Production of membrane proteins (MPs) is a challenging task as their hydrophobic nature
and their specific requirements in cellular expression systems frequently prevent an efficient …