Functional Mapping of PilF and PilQ in the Pseudomonas aeruginosa Type IV Pilus System

J Koo, T Tang, H Harvey, S Tammam… - Biochemistry, 2013 - ACS Publications
Pseudomonas aeruginosa uses type IV pili (T4P) to interact with the environment and as key
virulence factors when acting as an opportunistic pathogen. Assembly of the outer …

Pseudomonas aeruginosa minor pilins prime type IVa pilus assembly and promote surface display of the PilY1 adhesin

Y Nguyen, S Sugiman-Marangos, H Harvey… - Journal of Biological …, 2015 - ASBMB
Type IV pili (T4P) contain hundreds of major subunits, but minor subunits are also required
for assembly and function. Here we show that Pseudomonas aeruginosa minor pilins prime …

PilN binding modulates the structure and binding partners of the Pseudomonas aeruginosa type IVa pilus protein PilM

M McCallum, S Tammam, DJ Little, H Robinson… - Journal of biological …, 2016 - ASBMB
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that expresses type IVa
pili. The pilus assembly system, which promotes surface-associated twitching motility and …

PilF Is an Outer Membrane Lipoprotein Required for Multimerization and Localization of the Pseudomonas aeruginosa Type IV Pilus Secretin

J Koo, S Tammam, SY Ku, LM Sampaleanu… - Journal of …, 2008 - Am Soc Microbiol
ABSTRACT Type IV pili (T4P) are retractile appendages that contribute to the virulence of
bacterial pathogens. PilF is a Pseudomonas aeruginosa lipoprotein that is essential for T4P …

PilMNOPQ from the Pseudomonas aeruginosa type IV pilus system form a transenvelope protein interaction network that interacts with PilA

S Tammam, LM Sampaleanu, J Koo… - Journal of …, 2013 - Am Soc Microbiol
Pseudomonas aeruginosa type IV pili (T4P) are virulence factors that promote infection of
cystic fibrosis and immunosuppressed patients. As the absence of T4P impairs colonization …

Periplasmic domains of Pseudomonas aeruginosa PilN and PilO form a stable heterodimeric complex

LM Sampaleanu, JB Bonanno, M Ayers, J Koo… - Journal of molecular …, 2009 - Elsevier
Type IV pili (T4P) are bacterial virulence factors responsible for attachment to surfaces and
for twitching motility, a motion that involves a succession of pilus extension and retraction …

Conserved, unstructured regions in Pseudomonas aeruginosa PilO are important for type IVa pilus function

TL Leighton, MC Mok, MS Junop, PL Howell… - Scientific reports, 2018 - nature.com
Pseudomonas aeruginosa uses long, thin fibres called type IV pili (T4P) for adherence to
surfaces, biofilm formation, and twitching motility. A conserved subcomplex of PilMNOP is …

Pseudomonas aeruginosa minor pilins are incorporated into type IV pili

CL Giltner, M Habash, LL Burrows - Journal of molecular biology, 2010 - Elsevier
Type IV pili are long filamentous appendages required for both adhesion and a unique form
of motility known as twitching. Twitching motility involves the extension and retraction of the …

Structural and functional studies of the Pseudomonas aeruginosa minor pilin, PilE

Y Nguyen, H Harvey, S Sugiman-Marangos… - Journal of Biological …, 2015 - ASBMB
Many bacterial pathogens, including Pseudomonas aeruginosa, use type IVa pili (T4aP) for
attachment and twitching motility. T4aP are composed primarily of major pilin subunits …

PilM/N/O/P proteins form an inner membrane complex that affects the stability of the Pseudomonas aeruginosa type IV pilus secretin

M Ayers, LM Sampaleanu, S Tammam, J Koo… - Journal of molecular …, 2009 - Elsevier
The highly conserved pilM/N/O/P/Q gene cluster is among the core set of genes required for
cell surface expression of type IV pili and associated twitching motility. With the exception of …