IN the ultra-microspectrophotometer for high resolution the absorption spectra of biological
objects can be determined by utilizing the entire resolving power of the microscope. In order …

THE study of various problems with ultramicrospectrographic procedures very often
necessitates the determination of the total content of a whole cell of a certain light absorbing …

The design of a scanning and integrating microspectrophotometer for studying the
absorption of tissue cells in the wavelength region 2200 to 7000 A is described. The …

I It-N u ra microspectrophotometric work for quantitative purposes the measuring instruments
must fulfil a large number of requirements. These have been described earlier (1). Hitherto …

Light scattered from a microscopic object and thereby lost from an imageforming system can
be one of the major sources of error in a microspectrophotometric measurement. The …

SINCE Caspersson's pioneer work in the field of microspectroscopy, several complex
electrical and optical instruments have been devised in order to study the absorption of …

Because of the absence of suitable standards, microspectrophotometry suffers from a lack of
objective verification. An approach to this problem is suggested which is based on the …

CURVES giving the light transmission along a measuring line traversing a microscopic
object are obtained with the recording ultramicrospectrophotometers [l, 2, 4, 51. These …

~~ ICR~ SPECTR~ METRY is one of the most important methods for measuring the total
amount and distribution of substances in individual cells (eg 2, 3, 8). While in biochemistry …

In spite of a number of examples of the apparently successful application of the two-
wavelength method of microspectrophotometry to biological materials, a demonstration of …