A simple method for beta-glucosidase immobilization and its application in soybean isoflavone glycosides hydrolysis

S Hu, D Wang, J Hong - Biotechnology and bioprocess engineering, 2018 - Springer
In this study, a simple, inexpensive and fast β-glucosidase immobilization system was
constructed and evaluated in isoflavone glycosides hydrolysis. A β-glucosidase gene from …

Carbohydrate-binding module assisted purification and immobilization of β-glucosidase onto cellulose and application in hydrolysis of soybean isoflavone glycosides

F Chang, S Xue, X Xie, W Fang, Z Fang… - Journal of bioscience and …, 2018 - Elsevier
Complicated purification steps, together with the fact that β-glucosidase has to be tolerant to
ethanol restricts the application of β-glucosidase in isoflavone aglycone hydrolyzing …

Improve ethanol tolerance of β-glucosidase Bgl1A by semi-rational engineering for the hydrolysis of soybean isoflavone glycosides

W Fang, Y Yang, X Zhang, Q Yin, X Zhang… - Journal of …, 2016 - Elsevier
A β-glucosidase Bgl1A variant (A24S/F297Y) with improved ethanol tolerance was obtained
by semi-rational engineering. At 30–40° C, IC 50 values (the amount required for inhibiting …

Hydrolysis of soy isoflavone glycosides by recombinant β-glucosidase from hyperthermophile Thermotoga maritima

Y Xue, J Yu, X Song - Journal of Industrial Microbiology and …, 2009 - academic.oup.com
A recombinant Thermotoga maritima β-glucosidase A (BglA) was purified to homogeneity for
performing enzymatic hydrolysis of isoflavone glycosides from soy flour. The kinetic …

Characterization of yeast cell surface displayed Aspergillus oryzae β-glucosidase 1 high hydrolytic activity for soybean isoflavone

J Ito, H Sahara, M Kaya, Y Hata, S Shibasaki… - Journal of Molecular …, 2008 - Elsevier
The yeast Saccharomyces cerevisiae GRI-117-UK was transformed to either display or
secrete β-glucosidase 1 (BGL1) from the koji mold, Aspergillus oryzae. The β-glucosidase …

Comparison of three thermostable β-glucosidases for application in the hydrolysis of soybean isoflavone glycosides

X Song, Y Xue, Q Wang, X Wu - Journal of agricultural and food …, 2011 - ACS Publications
A novel thermostable β-glucosidase (Te-BglA) from Thermoanaerobacter ethanolicus
JW200 was cloned, characterized and compared for its activity against isoflavone glycosides …

[HTML][HTML] Hydrolysis of soybean isoflavones by Debaryomyces hansenii UFV-1 immobilised cells and free β-glucosidase

GP Maitan-Alfenas, LGA Lage, MN de Almeida… - Food chemistry, 2014 - Elsevier
An intracellular β-glucosidase from Debaryomyces hansenii UFV-1 was produced in an YP
medium with cellobiose as the carbon source. This enzyme was purified, characterised and …

[HTML][HTML] Characterization of β-glucosidase from Aspergillus terreus and its application in the hydrolysis of soybean isoflavones

F Yan, W Xia, X Zhang, S Chen, X Nie… - Journal of Zhejiang …, 2016 - ncbi.nlm.nih.gov
An extracellular β-glucosidase produced by Aspergillus terreus was identified, purified,
characterized and was tested for the hydrolysis of soybean isoflavone. Matrix-assisted laser …

Hydrolysis of isoflavone glycoside by immobilization of β-glucosidase on a chitosan-carbon in two-phase system

J Chang, YS Lee, SJ Fang, DJ Park, YL Choi - International journal of …, 2013 - Elsevier
We explored a method to examine the hydrolysis of isoflavone glycoside by immobilizing β-
glucosidase on chitosan-carbon beads in an aqueous–organic two-phase system. The …

Molecular cloning and characterization of a novel β-glucosidase with high hydrolyzing ability for soybean isoflavone glycosides and glucose-tolerance from soil …

G Li, Y Jiang, X Fan, Y Liu - Bioresource Technology, 2012 - Elsevier
A novel β-glucosidase (Bgl1269) was identified from a metagenomic library of mangrove soil
by activity-based functional screening. Sequence analysis revealed that Bgl1269 encodes a …