Y chromosome detection by Real Time PCR and pyrophosphorolysis‐activated polymerisation using free fetal DNA isolated from maternal plasma

EMJ Boon, HB Schlecht, P Martin… - … in Affiliation With the …, 2007 - Wiley Online Library
Objectives To validate the use of Real Time PCR, a widely used technique that can detect
very low levels of Y chromosomal sequence, and to assess the use of a highly sensitive …

Detection of Y chromosome‐specific DNA in the plasma and urine of pregnant women using nested polymerase chain reaction

MK Al‐Yatama, AS Mustafa, S Ali, S Abraham… - Prenatal …, 2001 - Wiley Online Library
The present study was undertaken to evaluate a nested polymerase chain reaction (PCR)
for detection of Y chromosome‐specific fetal DNA in maternal plasma and urine of pregnant …

Early non-invasive detection of fetal Y chromosome sequences in maternal plasma using multiplex PCR

A Kolialexi, G Tounta, P Apostolou, C Vrettou… - European Journal of …, 2012 - Elsevier
OBJECTIVE: Clinical indications for fetal sex determination include risk of X-linked
disorders, a family history of conditions associated with ambiguous development of the …

Analysis of multiple loci can increase reliability of detection of fetal Y‐chromosome DNA in maternal plasma

J Wagner, S Džijan, D Pavan‐Jukić… - … in Affiliation With the …, 2008 - Wiley Online Library
Objective Aiming to develop more reliable methods for determination of fetal gender from
maternal plasma we compared three different systems of polymerase chain reaction (PCR) …

Replicate real‐time PCR testing of DNA in maternal plasma increases the sensitivity of non‐invasive fetal sex determination

I Hromadnikova, B Houbova… - … in Affiliation With the …, 2003 - Wiley Online Library
Background We determined fetal sex in pregnancies referred for invasive prenatal diagnosis
procedures by analysis of DNA in maternal plasma. Methods Twelve pregnancies at risk of X …

Multiple testing in fetal gender determination from maternal blood by polymerase chain reaction

F Lagona, M Smid, N Papasergio, A Ferrari, M Ferrari… - Human genetics, 1998 - Springer
Fetal male DNA can be identified in maternal blood by polymerase chain reaction (PCR)
amplification of Y-specific sequences. This technology has not reached a satisfactory …

Non-invasive fetal sex determination using a conventional nested PCR analysis of fetal DNA in maternal plasma

W Tungwiwat, G Fucharoen, T Ratanasiri… - Clinica Chimica …, 2003 - Elsevier
Background: In order to provide a reliable non-invasive method for fetal sex determination in
a routine setting, we evaluated the possibility of identifying the fetal Y chromosome-specific …

Non‐invasive diagnosis of fetal sex; utilisation of free fetal DNA in maternal plasma and ultrasound

ND Avent, LS Chitty - … in Affiliation With the International Society …, 2006 - Wiley Online Library
Non-invasive prenatal diagnosis is now a clinical reality, using both early ultrasound and
molecular DNA methods. Technical advances in the sensitivity of the polymerase chain …

Fetal sex determination with real time PCR of fetal DNA in maternal plasma

L Lázár, Z Bán, O Szakács, B Nagy, A Beke… - Orvosi …, 2003 - europepmc.org
INTRODUCTION: Non-invasive methods using maternal plasma and serum for molecular
genetic diagnosis become an important field of interest in prenatal genetic diagnosis. Free …

A new Y chromosome marker for noninvasive fetal gender determination

OB Vainer, AV Katokhin, SM Kustov… - Annals of the New …, 2008 - Wiley Online Library
We have developed a real‐time PCR assay for early fetal gender diagnostics based on the
analysis of the Y chromosome–specific multicopy DAZ sequence in the circulating DNA from …