In vivo imaging of intact Drosophila larvae at sub-cellular resolution

Y Zhang, P Füger, SB Hannan, JV Kern… - JoVE (Journal of …, 2010 - jove.com
Recent improvements in optical imaging, genetically encoded fluorophores and genetic
tools allowing efficient establishment of desired transgenic animal lines have enabled …

[HTML][HTML] LarvaSPA, a method for mounting Drosophila larva for long-term time-lapse imaging

H Ji, C Han - Journal of visualized experiments: JoVE, 2020 - ncbi.nlm.nih.gov
Live imaging is a valuable approach for investigating cell biology questions. The Drosophila
larva is particularly suited for in vivo live imaging because the larval body wall and most …

Upright imaging of Drosophila embryos

M Belu, M Javier, K Ayasoufi, S Frischmann… - JoVE (Journal of …, 2010 - jove.com
Several well-known morphogenetic gradients and cellular movements occur along the
dorsal/ventral axis of the Drosophila embryo. However, the current techniques used to view …

Long-term in vivo imaging of Drosophila larvae

P Kakanj, SA Eming, L Partridge, M Leptin - Nature Protocols, 2020 - nature.com
The Drosophila larva has been used to investigate many processes in cell biology, including
morphogenesis, physiology and responses to drugs and new therapeutic compounds …

Live imaging of synapse development and measuring protein dynamics using two-color fluorescence recovery after photo-bleaching at Drosophila synapses

P Füger, LB Behrends, S Mertel, SJ Sigrist… - Nature protocols, 2007 - nature.com
Here we describe how to anesthetize and image Drosophila larvae as to follow'the life
history'of identified synapses and synaptic components. This protocol is sensitive, for …

Preparing individual Drosophila egg chambers for live imaging

TT Weil, RM Parton, I Davis - JoVE (Journal of Visualized Experiments), 2012 - jove.com
Live cell imaging is an important technique applied to a number of Drosophila tissues used
as models to investigate topics such as axis specification, cell differentiation and …

Chloroform and desflurane immobilization with recovery of viable Drosophila larvae for confocal imaging

D Cevik, M Acker, P Arefi, R Ghaemi, J Zhang… - Journal of insect …, 2019 - Elsevier
Imaging of living, intact Drosophila larvae is challenged if normal bodily function must be
observed or when healthy larvae must be recovered for subsequent studies. Here, we …

Preparation of developing and adult Drosophila brains and retinae for live imaging

WR Williamson, PR Hiesinger - JoVE (Journal of Visualized Experiments), 2010 - jove.com
The Drosophila brain and visual system are widely utilized model systems to study neuronal
development, function and degeneration. Here we show three preparations of the brain and …

Applications of immobilization of drosophila tissues with fibrin clots for live imaging

J Januschke, N Loyer - JoVE (Journal of Visualized Experiments), 2020 - jove.com
Drosophila is an important model system to study a vast range of biological questions.
Various organs and tissues from different developmental stages of the fly such as imaginal …

Dissecting, fixing, and visualizing the Drosophila pupal notum

JS White, KS LaFever, A Page-McCaw - JoVE (Journal of Visualized …, 2022 - jove.com
The pupae of Drosophila melanogaster are immobile for several days during
metamorphosis, during which they develop a new body with a thin transparent adult …