In vivo imaging of intact Drosophila larvae at sub-cellular resolution
Y Zhang, P Füger, SB Hannan, JV Kern… - JoVE (Journal of …, 2010 - jove.com
Recent improvements in optical imaging, genetically encoded fluorophores and genetic
tools allowing efficient establishment of desired transgenic animal lines have enabled …
tools allowing efficient establishment of desired transgenic animal lines have enabled …
[HTML][HTML] LarvaSPA, a method for mounting Drosophila larva for long-term time-lapse imaging
H Ji, C Han - Journal of visualized experiments: JoVE, 2020 - ncbi.nlm.nih.gov
Live imaging is a valuable approach for investigating cell biology questions. The Drosophila
larva is particularly suited for in vivo live imaging because the larval body wall and most …
larva is particularly suited for in vivo live imaging because the larval body wall and most …
Upright imaging of Drosophila embryos
M Belu, M Javier, K Ayasoufi, S Frischmann… - JoVE (Journal of …, 2010 - jove.com
Several well-known morphogenetic gradients and cellular movements occur along the
dorsal/ventral axis of the Drosophila embryo. However, the current techniques used to view …
dorsal/ventral axis of the Drosophila embryo. However, the current techniques used to view …
Long-term in vivo imaging of Drosophila larvae
P Kakanj, SA Eming, L Partridge, M Leptin - Nature Protocols, 2020 - nature.com
The Drosophila larva has been used to investigate many processes in cell biology, including
morphogenesis, physiology and responses to drugs and new therapeutic compounds …
morphogenesis, physiology and responses to drugs and new therapeutic compounds …
Live imaging of synapse development and measuring protein dynamics using two-color fluorescence recovery after photo-bleaching at Drosophila synapses
P Füger, LB Behrends, S Mertel, SJ Sigrist… - Nature protocols, 2007 - nature.com
Here we describe how to anesthetize and image Drosophila larvae as to follow'the life
history'of identified synapses and synaptic components. This protocol is sensitive, for …
history'of identified synapses and synaptic components. This protocol is sensitive, for …
Preparing individual Drosophila egg chambers for live imaging
TT Weil, RM Parton, I Davis - JoVE (Journal of Visualized Experiments), 2012 - jove.com
Live cell imaging is an important technique applied to a number of Drosophila tissues used
as models to investigate topics such as axis specification, cell differentiation and …
as models to investigate topics such as axis specification, cell differentiation and …
Chloroform and desflurane immobilization with recovery of viable Drosophila larvae for confocal imaging
Imaging of living, intact Drosophila larvae is challenged if normal bodily function must be
observed or when healthy larvae must be recovered for subsequent studies. Here, we …
observed or when healthy larvae must be recovered for subsequent studies. Here, we …
Preparation of developing and adult Drosophila brains and retinae for live imaging
WR Williamson, PR Hiesinger - JoVE (Journal of Visualized Experiments), 2010 - jove.com
The Drosophila brain and visual system are widely utilized model systems to study neuronal
development, function and degeneration. Here we show three preparations of the brain and …
development, function and degeneration. Here we show three preparations of the brain and …
Applications of immobilization of drosophila tissues with fibrin clots for live imaging
J Januschke, N Loyer - JoVE (Journal of Visualized Experiments), 2020 - jove.com
Drosophila is an important model system to study a vast range of biological questions.
Various organs and tissues from different developmental stages of the fly such as imaginal …
Various organs and tissues from different developmental stages of the fly such as imaginal …
Dissecting, fixing, and visualizing the Drosophila pupal notum
JS White, KS LaFever, A Page-McCaw - JoVE (Journal of Visualized …, 2022 - jove.com
The pupae of Drosophila melanogaster are immobile for several days during
metamorphosis, during which they develop a new body with a thin transparent adult …
metamorphosis, during which they develop a new body with a thin transparent adult …