Characterization of a metagenome-derived halotolerant cellulase

S Voget, HL Steele, WR Streit - Journal of biotechnology, 2006 - Elsevier
Metagenomes of uncultured microorganisms represent a sheer unlimited resource for
discovery of novel biocatalysts. Here, we report on the biochemical characterisation of a …

Biochemical and structural characterization of a novel halotolerant cellulase from soil metagenome

R Garg, R Srivastava, V Brahma, L Verma… - Scientific reports, 2016 - nature.com
Cellulase catalyzes the hydrolysis of β-1, 4-linkages of cellulose to produce industrially
relevant monomeric subunits. Cellulases find their applications in pulp and paper, laundry …

Discovery of new cellulases from the metagenome by a metagenomics-guided strategy

C Yang, Y Xia, H Qu, AD Li, R Liu, Y Wang… - Biotechnology for …, 2016 - Springer
Background Energy shortage has become a global problem. Production of biofuels from
renewable biomass resources is an inevitable trend of sustainable development. Cellulose …

Mining metagenomes for novel cellulase genes

CJ Duan, JX Feng - Biotechnology letters, 2010 - Springer
Abstract Cellulases hydrolyze the β-1, 4 linkages of cellulose and are widely used in food,
brewing and wine, animal feed, textiles and laundry, and pulp and paper industries …

Identification and characterization of novel cellulolytic and hemicellulolytic genes and enzymes derived from German grassland soil metagenomes

H Nacke, M Engelhaupt, S Brady, C Fischer, J Tautzt… - Biotechnology …, 2012 - Springer
Soil metagenomes represent an unlimited resource for the discovery of novel biocatalysts
from soil microorganisms. Three large-inserts metagenomic DNA libraries were constructed …

Expression and characterization of a novel metagenome-derived cellulase Exo2b and its application to improve cellulase activity in Trichoderma reesei

A Geng, G Zou, X Yan, Q Wang, J Zhang, F Liu… - Applied microbiology …, 2012 - Springer
A metagenomic fosmid library containing 1× 10 5 clones was constructed from a biogas
digester fed with pig ordure and rice straw. In total, 121 clones with activity of 4 …

Discovery and characterization of a thermostable and highly halotolerant GH5 cellulase from an icelandic hot spring isolate

D Zarafeta, D Kissas, C Sayer, SR Gudbergsdottir… - PLoS …, 2016 - journals.plos.org
With the ultimate goal of identifying robust cellulases for industrial biocatalytic conversions,
we have isolated and characterized a new thermostable and very halotolerant GH5 …

Upgrading the enzymatic hydrolysis of lignocellulosic biomass by immobilization of metagenome-derived novel halotolerant cellulase on the carboxymethyl cellulose …

E Motamedi, SF Sadeghian Motahar, M Maleki… - Cellulose, 2021 - Springer
Degradation of various types of lignocellulosic biomass that require harsh pretreatment and
abnormal operating conditions often occur in presence of a large amount of salt. Therefore …

Identification of cellulase genes from the metagenomes of compost soils and functional characterization of one novel endoglucanase

H Pang, P Zhang, CJ Duan, XC Mo, JL Tang… - Current microbiology, 2009 - Springer
Metagenomics, a new research field developed over the past decade, aims to identify
potential enzymes from nonculturable microbes. In this study, genes encoding three …

Cellulases from thermophiles found by metagenomics

JJ Escuder-Rodríguez, ME DeCastro, ME Cerdán… - Microorganisms, 2018 - mdpi.com
Cellulases are a heterogeneous group of enzymes that synergistically catalyze the
hydrolysis of cellulose, the major component of plant biomass. Such reaction has …