Background Since the introduction of what became today's standard for cryo-embedding of
biological macromolecules at native conditions more than 30 years ago, techniques and …

Cryo‐electron microscopy (cryo‐EM) is a structural biological method that is used to
determine the 3D structures of biomacromolecules. After years of development, cryo‐EM has …

For many years, structure determination of biological macromolecules by cryo-electron
microscopy (cryo-EM) was limited to large complexes or low-resolution models. With recent …

Single particle cryo-electron microscopy (cryo-EM) has matured into a robust method for the
determination of biological macromolecule structures in the past decade, complementing X …

Cryo-electron microscopy (cryo-EM) is a structural molecular and cellular biology technique
that has experienced major advances in recent years. Technological developments in image …

Macromolecular machines carry out many cellular functions. Cryo-electron microscopy (cryo-
EM) is emerging as a powerful method for studying the structure, assembly and dynamics of …

Time-resolved cryo-electron microscopy (cryo-EM) combines the known advantages of
single-particle cryo-EM in visualizing molecular structure with the ability to dissect the time …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Cryo‐electron microscopy (cryo‐EM) is increasingly becoming a mainstream technology for
studying the architecture of cells, viruses and protein assemblies at molecular resolution …

▪ Abstract Cryo-electron microscopy (cryo-EM) of biological molecules in single-particle (ie,
unordered, nonaggregated) form is a new approach to the study of molecular assemblies …