Amplification and application of the HMG box of bovine SRY gene for sex determination

W Lu, N Rawlings, J Zhao, H Wang - Animal reproduction science, 2007 - Elsevier
A fast and reliable method for bovine sexing has been developed through amplification of
the bovine high motility group (HMG) box of the sex-determining region of the Y …

Amplification of porcine SRY gene for sex determination

SG Choi, MS Bae, ES Lee, SO Kim, BK Kim… - … journal of animal …, 2009 - koreascience.kr
The separation of X and Y chromosome-bearing sperm is of use in many aspects of livestock
maintenance. In this study, we sought to determine the difference in DNA content between X …

Sex determination in goat by amplification of the HMG box using duplex PCR

L Shi, W Yue, Y Ren, F Lei, J Zhao - Animal reproduction science, 2008 - Elsevier
The objective of this study was to obtain a fast, accurate and reliable method of determining
the sex of goat embryos prior to implantation through amplification of the high-motility-group …

A novel repeated sequence located on the bovine Y chromosome: its application to rapid and precise embryo sexing by PCR

S Kageyama, I Yoshida, K Kawakura… - Journal of Veterinary …, 2004 - jstage.jst.go.jp
A novel repeated sequence specific to male cattle was identified and named S4. S4 is a
highly repetitive sequence and is a 1.5 kb repeating unit that contains various internal …

Molecular cloning and expression of buffalo SRY gene: A mammalian sex determination protein

AM Shende, VHM Kutty, SS Ramteke, SK Ghosh… - 2014 - nopr.niscpr.res.in
A number of proteins are expressed specifically in X-and Y-chromosome bearing
spermatozoa. The Sry protein is known to be expressed only in Y-chromosome bearing …

Bovine sex determining region Y: cloning, optimized expression, and purification

B Soleymani, SH Hafezian, GR Mianji… - Animal …, 2017 - Taylor & Francis
Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein
with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was …

A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes

ACY Phua, RB Abdullah, Z Mohamed - Journal of Reproduction and …, 2003 - jstage.jst.go.jp
Sex determination of livestock is performed to achieve the objectives of livestock breeding
programmes. Techniques for sex determination have evolved from karyotyping to detecting …

PCR-sexing of bovine embryos: a simplified protocol

P Bredbacka, A Kankaanpää, J Peippo - Theriogenology, 1995 - Elsevier
To make bovine embryo sexing under farm conditions more feasible we developed a
simplified protocol utilizing manual biopsy and detection of the Y chromosome directly from …

Production of monoclonal antibody against recombinant bovine sex‐determining region Y (SRY) and their preferential binding to Y chromosome‐bearing sperm

B Soleymani, K Mansouri… - … in Domestic Animals, 2021 - Wiley Online Library
Separation of X and Y chromosome‐bearing sperm is an appropriate method for the
selection of desired sex of offspring to increase the profit in livestock industries. The purpose …

Development of Y‐chromosome‐Specific SCAR Markers Conserved in Taurine, Zebu and Bubaline Cattle

BCA Alves, VFM Hossepian de Lima… - … in domestic animals, 2010 - Wiley Online Library
Contents Sex pre‐selection of bovine offsprings has commercial relevance for cattle
breeders and several methods have been used for embryo sex determination. Polymerase …