Differential inducibility of human and porcine dental pulp-derived cells into odontoblasts

A Tonomura, Y Sumita, Y Ando, D Iejima… - Connective tissue …, 2007 - Taylor & Francis
A robust method for generating odontoblasts from cultured dental pulp cells has not been
established. In this study, efficient methods for deriving odontoblasts from cultured human …

Rat osteoblast cultures

IR Orriss, SEB Taylor, TR Arnett - Bone research protocols, 2012 - Springer
This chapter describes the isolation, culture and staining of primary osteoblasts from the
calvaria and long bones of neonatal rats. The key advantages of this assay are that it allows …

Bone regeneration and stem cells

K Arvidson, BM Abdallah, LA Applegate… - Journal of cellular …, 2011 - Wiley Online Library
Introduction• Bone fracture healing and healing problems• Biomaterial scaffolds and tissue
engineering in bone formation‐Bone tissue engineering‐Biomaterial scaffolds‐Synthetic …

Cell lines and primary cell cultures in the study of bone cell biology

V Kartsogiannis, KW Ng - Molecular and cellular endocrinology, 2004 - Elsevier
Bone is a metabolically active and highly organized tissue consisting of a mineral phase of
hydroxyapatite and amorphous calcium phosphate crystals deposited in an organic matrix …

Biomineralization process in hard tissues: The interaction complexity within protein and inorganic counterparts

V Sharma, A Srinivasan, F Nikolajeff, S Kumar - Acta biomaterialia, 2021 - Elsevier
Biomineralization can be considered as nature's strategy to produce and sustain
biominerals, primarily via creation of hard tissues for protection and support. This review …

Bone tissue engineering by primary osteoblast-like cells in a monolayer system and 3-dimensional collagen gel

HP Wiesmann, N Nazer, C Klatt, T Szuwart… - Journal of oral and …, 2003 - Elsevier
PURPOSE:: To engineer living bone tissue in vitro, bone cells must be multiplied and
differentiated in cell culture. Osteoblasts are known to be the crucial cells responsible for the …

Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, β-glycerophosphate and dexamethasone on osteoblastic differentiation

MJ Coelho, MH Fernandes - Biomaterials, 2000 - Elsevier
This work analyses the proliferation/differentiation behaviour of human bone marrow cells
cultured in α-minimum essential medium supplemented with 10% foetal bovine serum …

Gene expression, signal transduction and tissue-specific biomineralization during mammalian tooth development.

HC Slavkin, CC Hu, Y Sakakura… - Critical Reviews in …, 1992 - europepmc.org
Tooth development provides a paradigm for intrinsic molecular controls for cell-and
extracellular matrix (ECM)-mediated biomineralization. The intent of this review is to …

Ex vivo organ cultures as models to study bone biology

T Bellido, J Delgado Calle - … of Bone and Mineral Research Plus, 2020 - academic.oup.com
The integrity of the skeleton is maintained by the coordinated and balanced activities of the
bone cells. Osteoclasts resorb bone, osteoblasts form bone, and osteocytes orchestrate the …

Potential of biomimetic surfaces to promote in vitro osteoblast-like cell differentiation

S Hattar, A Asselin, D Greenspan, M Oboeuf, A Berdal… - Biomaterials, 2005 - Elsevier
Bioactive glasses, osteoproductive materials, have received considerable attention as bone
graft substitutes in the treatment of bony defects. More recent strategies for achieving a …