Best practices in metabarcoding of fungi: from experimental design to results
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …
Illumina metabarcoding of a soil fungal community
PA Schmidt, M Bálint, B Greshake, C Bandow… - Soil Biology and …, 2013 - Elsevier
Next generation metabarcoding is becoming an indispensable tool in fungal community
ecology. Here we tested Illumina metabarcoding, a method that generates shorter reads but …
ecology. Here we tested Illumina metabarcoding, a method that generates shorter reads but …
Scaling up discovery of hidden diversity in fungi: impacts of barcoding approaches
The fungal kingdom is a hyperdiverse group of multicellular eukaryotes with profound
impacts on human society and ecosystem function. The challenge of documenting and …
impacts on human society and ecosystem function. The challenge of documenting and …
GlobalFungi, a global database of fungal occurrences from high-throughput-sequencing metabarcoding studies
Fungi are key players in vital ecosystem services, spanning carbon cycling, decomposition,
symbiotic associations with cultivated and wild plants and pathogenicity. The high …
symbiotic associations with cultivated and wild plants and pathogenicity. The high …
Towards PacBio‐based pan‐eukaryote metabarcoding using full‐length ITS sequences
L Tedersoo, S Anslan - Environmental microbiology reports, 2019 - Wiley Online Library
Development of high‐throughput sequencing techniques has greatly benefited our
understanding about microbial ecology, yet the methods producing short reads suffer from …
understanding about microbial ecology, yet the methods producing short reads suffer from …
Daring to be differential: metabarcoding analysis of soil and plant-related microbial communities using amplicon sequence variants and operational taxonomical units
Background Microorganisms are not only indispensable to ecosystem functioning, they are
also keystones for emerging technologies. In the last 15 years, the number of studies on …
also keystones for emerging technologies. In the last 15 years, the number of studies on …
Polymerase matters: non-proofreading enzymes inflate fungal community richness estimates by up to 15%
Rare taxa overwhelm metabarcoding data generated using next-generation sequencing
(NGS). Low frequency Operational Taxonomic Units (OTUs) may be artifacts generated by …
(NGS). Low frequency Operational Taxonomic Units (OTUs) may be artifacts generated by …
[HTML][HTML] Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding
Along with recent developments in high-throughput sequencing (HTS) technologies and
thus fast accumulation of HTS data, there has been a growing need and interest for …
thus fast accumulation of HTS data, there has been a growing need and interest for …
[PDF][PDF] Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi
Rapid development of high-throughput (HTS) molecular identification methods has
revolutionized our knowledge about taxonomic diversity and ecology of fungi. However …
revolutionized our knowledge about taxonomic diversity and ecology of fungi. However …
Sequence-based classification and identification of Fungi
Fungal taxonomy and ecology have been revolutionized by the application of molecular
methods and both have increasing connections to genomics and functional biology …
methods and both have increasing connections to genomics and functional biology …