An improved protocol for genetic transformation of juvenile explants of Carrizo (Citrus
sinensis Osb.× Poncirus trifoliata L. Raf.), Duncan (Citrus paradisi Macf.), Hamlin (Citrus …

A procedure for increased efficiency of production of transgenic citrus plants was developed
by extending the exposure of the explants to the selection agent and by grafting in vitro the …

Genetic transformation of many plant species relies on in vitro tissue culture-based
approaches. This can be a labor-intensive process, requiring aseptic conditions and …

ABSTRACT A Mature Citrus Facility (MCF) was established at the University of Florida's
Citrus Research and Education Center (CREC) to produce transgenic citrus (Citrus spp.) …

Citrus is one important fruit crop in China and worldwide. Genetic transformation with more
recently cloned target genes is an effective alternative for citrus improvement. In the past …

Background Citrus has an extended juvenile phase and trees can take 2–20 years to
transition to the adult reproductive phase and produce fruit. For citrus variety development …

Transformation and high efficient regeneration of transgenic plants from embryogenic
calluses of Bingtang sweet orange [Citrus sinensis (L.) Osbeck] was reported. Embryogenic …

Citrus is the most important fruit tree crop in the world, with a production of more than 100
million tons annually. The area of origin of Citrus is believed to be southeastern Asia, where …

Since the initial reports on production of transgenic Citrus via Agrobacterium-mediated
transformation, significant progress has been made, and many steps of this procedure using …

Key message Regulatory sequences from the citrus constitutive genes cyclophilin (CsCYP),
glyceraldehyde-3-phosphate dehydrogenase C2 (CsGAPC2), and elongation factor 1-alpha …