The development and optimization of efficient transformation protocols is essential in new
citrus breeding programs, not only for rootstock, but also for scion improvement …

Citrus is one of the top fruit crops worldwide. Citrus production faces many challenges such
as diseases, insects, and abiotic stresses. Genetic improvement of citrus using conventional …

The presence of marker genes conferring antibiotic resistance in transgenic plants
represents a serious obstacle for their public acceptance and future commercialization. In …

Background There had been many reports on genetic transformation of Citrus for functional
genomic studies but few included genes associated with flower or fruit traits. A major reason …

A new binary vector (pT-LAB21) that contains a modification of the Aequorea victoria green
fluorescent protein-GFP (erGFP7 INT) as a reporter gene was constructed for the use in …

The effects of a maize knotted1 (kn1) gene under the control of the cauliflower mosaic virus
35S promoter on genetic transformation efficiencies of six citrus genotypes were tested. The …

In order to improve transformation efficiency in citrus two green fluorescent protein (GFP)
constructions, an endoplasmic reticulum targeted (GFP-ER) and cytoplasmic targeted (Cy …

In this study, conditions were optimized for transient gene expression in Rough Lemon
(Citrus jambhiri Lush.), a major rootstock used in the citrus growing regions of Pakistan …

Procedures for high efficiency production of transgenic citrus plants using an Agrobacterium
tumefaciens system with plasmolysis treatment were developed. Longitudinally cut epicotyl …

A method for Agrobacterium-mediated transformation of Citrus and organogenic
regeneration of transgenic plants is reported. Internodal stem segments were co-cultured …