Stem-cell-derived human microglia transplanted into mouse brain to study human disease

N Fattorelli, A Martinez-Muriana, L Wolfs, I Geric… - Nature protocols, 2021 - nature.com
Microglia are critically involved in complex neurological disorders with a strong genetic
component, such as Alzheimer's disease, Parkinson's disease and frontotemporal dementia …

Generating microglia from human pluripotent stem cells: novel in vitro models for the study of neurodegeneration

AM Speicher, H Wiendl, SG Meuth… - Molecular …, 2019 - Springer
Microglia play an essential role for central nervous system (CNS) development and
homeostasis and have been implicated in the onset, progression, and clearance of …

Human iPSC-derived microglia assume a primary microglia-like state after transplantation into the neonatal mouse brain

DS Svoboda, MI Barrasa, J Shu… - Proceedings of the …, 2019 - National Acad Sciences
Microglia are essential for maintenance of normal brain function, with dysregulation
contributing to numerous neurological diseases. Protocols have been developed to derive …

Efficient derivation of microglia-like cells from human pluripotent stem cells

J Muffat, Y Li, B Yuan, M Mitalipova, A Omer… - Nature medicine, 2016 - nature.com
Microglia, the only lifelong resident immune cells of the central nervous system (CNS), are
highly specialized macrophages that have been recognized to have a crucial role in …

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells

H Pandya, MJ Shen, DM Ichikawa, AB Sedlock… - Nature …, 2017 - nature.com
Microglia are resident inflammatory cells of the CNS and have important roles in
development, homeostasis and a variety of neurologic and psychiatric diseases. Difficulties …

Development and validation of a simplified method to generate human microglia from pluripotent stem cells

A McQuade, M Coburn, CH Tu, J Hasselmann… - Molecular …, 2018 - Springer
Background Microglia, the principle immune cells of the brain, play important roles in
neuronal development, homeostatic function and neurodegenerative disease. Recent …

Single-cell transcriptomics defines an improved, validated monoculture protocol for differentiation of human iPSC to microglia

SJ Washer, M Perez-Alcantara, Y Chen, J Steer… - Scientific Reports, 2022 - nature.com
There is increasing genetic evidence for the role of microglia in neurodegenerative
diseases, including Alzheimer's, Parkinson's, and motor neuron disease. Therefore, there is …

Human iPSC-derived mature microglia retain their identity and functionally integrate in the chimeric mouse brain

R Xu, X Li, AJ Boreland, A Posyton, K Kwan… - Nature …, 2020 - nature.com
Microglia, the brain-resident macrophages, exhibit highly dynamic functions in
neurodevelopment and neurodegeneration. Human microglia possess unique features as …

A highly efficient human pluripotent stem cell microglia model displays a neuronal-co-culture-specific expression profile and inflammatory response

W Haenseler, SN Sansom, J Buchrieser, SE Newey… - Stem cell reports, 2017 - cell.com
Microglia are increasingly implicated in brain pathology, particularly neurodegenerative
disease, with many genes implicated in Alzheimer's, Parkinson's, and motor neuron disease …

Concise review: modeling neurodegenerative diseases with human pluripotent stem cell-derived microglia

W Haenseler, L Rajendran - Stem Cells, 2019 - academic.oup.com
Inflammation of the brain and the consequential immunological responses play pivotal roles
in neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease …