Relaxing constraints on data acquisition and position detection for trap stiffness calibration in optical tweezers

B Melo, F Almeida, G Temporão, T Guerreiro - Optics Express, 2020 - opg.optica.org
… Finally, still motivated by the interest in reducing the cost and complexity of an optical tweezer
… of an optical tweezer. We close with a brief discussion and the conclusions of this work. …

[HTML][HTML] Optical tweezers in single-molecule biophysics

CJ Bustamante, YR Chemla, S Liu… - Nature Reviews Methods …, 2021 - nature.com
Optical tweezers have become the method of choice in single-molecule manipulation studies.
In this Primer, we first review the physical principles of optical tweezersoptical tweezers

[HTML][HTML] Interrogating biology with force: single molecule high-resolution measurements with optical tweezers

M Capitanio, FS Pavone - Biophysical journal, 2013 - cell.com
… of the microsecond-scale relaxation times of biological … optical tweezers’ spatial and
temporal resolution down to today’s values, discussing the experimental variables and constraints

[HTML][HTML] Optical tweezers: theory and practice

G Pesce, PH Jones, OM Maragò, G Volpe - The European Physical …, 2020 - Springer
… theory of optical forces … in optical traps and its implementation in optical tweezers simulations.
Finally, we provide a general description of typical experimental setups of optical tweezers

Optical tweezers and their applications

P Polimeno, A Magazzu, MA Iati, F Patti, R Saija… - Journal of Quantitative …, 2018 - Elsevier
… While a comprehensive overview of numerical methods for optical tweezers it is beyond the
scope of this work, some cover of the topic can be found elsewhere [56], [57], [58], [59]. Here …

[PDF][PDF] Nonlinear elastic and viscoelastic deformation of the human red blood cell with optical tweezers

JP Mills, L Qie, M Dao, CT Lim… - Molecular & …, 2004 - oww-files-public.s3.amazonaws.com
… In addition, the values of elastic properties and characteristic time for viscoelastic relaxation
extracted from the present optical tweezers experiments on normal human red blood cells …

[HTML][HTML] Spectrin-level modeling of the cytoskeleton and optical tweezers stretching of the erythrocyte

J Li, M Dao, CT Lim, S Suresh - Biophysical journal, 2005 - cell.com
… Subsequently, direct stretching by optical tweezers of the … large deformation of RBC with
optical tweezers. We find that the … undergoes constant remodeling to always relax the in-plane …

Optical tweezers in single-molecule experiments

A Zaltron, M Merano, G Mistura, C Sada… - The European Physical …, 2020 - epjplus.epj.org
relaxed) molecules and torsionally constrained ones, even if it occurs at different force values.
In torsionally relaxed … explain the influence of torsional constraints on the structural stability …

[HTML][HTML] Force unfolding kinetics of RNA using optical tweezers. II. Modeling experiments

M Manosas, JD Wen, PTX Li, SB Smith, C Bustamante… - Biophysical journal, 2007 - cell.com
… Here, we introduce a mesoscopic model to analyze the hopping kinetics of RNA hairpins in
an optical tweezers setup. The model includes different elements of the experimental setup (…

Towards absolute calibration of optical tweezers

NB Viana, MS Rocha, ON Mesquita, A Mazolli… - Physical Review E …, 2007 - APS
… with a digital correlator, one obtains the Brownian relaxation time of the bead see Fig. 14 .
… 15 by the beam aperture constraint. Our measurement accuracy is not sufficient to verify the …