… Cerebellar granule neurons undergo apoptosis when deprived of chronic depolarization;
serum … Here we report that serum removal triggers cell injury, which is characterized by signs of …

… in sympathetic neurons. Our data show that removal of both K + and serum is important for
triggering PCD in this granule cell model. Furthermore, we have defined two populations of …

… Fire polished glass pipettes can be used, but we find that serum … Remove the suspended
cells (careful not to remove the … This step will remove large non-neuronal cells and provides …

… To determine whether c-Jun ala could block apoptosis in granule neurons, cells were
transfected with c-Jun ala and then were induced to die by removing serum and lowering the …

… In a recent study, the removal of serum was reported not to result in the activation of either
… In our freshly-isolated neuronal cultures, serum withdrawal resulted in a small but significant …

… after removing meninges from day 4 neonatal rat hippocampus. Dentate granule neurons
were isolated with trypsin digestion similar to that described for cerebellar granule neurons. …

… CEP-1347 saving in cerebellar granule neurons 24 h after potassium–serum deprivation. p
< … If trophic support is removed, pro-survival signaling is lost, but if subsequent JNK activation …

… Cerebellar granule neurons can be maintained in culture in a medium containing high
serum and depolarising levels of KCl. When serum is removed and the KCl levels lowered from …

… Removal of serum from primary cultures of cerebellar granule neurons induces oxidative
stress and … receptors blocks apoptosis of cultured cerebellar granule neurons. Mol. Pharmacol. …

… and superficial vasculature were removed under a dissecting … contains approximately 95%
granule neurons. We found that … medium with 10% fetal calf serum and incubated for 1 hr at …