… ], giving a global representation of the expression changes across multiple conditions. DGE
… , such as mean gene expression across replicates for each sample, log 2 fold-change (lfc) …

… , is RNA-seq data from the James Thomson Lab at the Morgridge Institute for Research. We
evaluated gene expression … RNA-seq experiments to study gene expression changes over …

… using a log 2 expression change cutoff of 0.5 (1.4 × expression change measured by qRT-…
-PCR expression changes, which define sets of differentially expressed genes at increasing …

… gene expression from RNA-seq data work by counting the sequencing library fragments that
map to the exons of each gene and dividing the count for each gene … of expression changes …

… To begin to rectify this situation, Illumina RNA-Seq was used to perform massively parallel
sequencing of three cDNA samples derived from P. capsici mycelia (MY), zoospores (ZO) and …

… of trimming on gene expression, we generated RNA-Seq data … the original, untrimmed gene
expression estimates to those … We found that the majority of the expression changes could …

… changes occur in mosquitoes after blood meals, and these are related to digestive and
reproductive processes, among others. Studies of these changes … changes in gene expression …

… change) algorithm to produce biologically meaningful rankings of differentially expressed
genes from RNA-seq … In general, we believe that the ranking of gene expression changes by …

… to seeing transcriptional changes in the current study, the GSEA and GeneGo expression
analysis make the most of identifying context for transcriptional expression changes, while the …

… cholerae gene expression. Collectively, our findings indicate that RNA-seq is a powerful
tool that can enable monitoring of pathogen gene expression during infection. …