A real‐time (TaqMan) PCR assay to differentiate Monilinia fructicola from other brown rot fungi of fruit crops

IR Van Brouwershaven, ML Bruil… - Plant …, 2010 - Wiley Online Library
… In this study an automated duplex real-time TaqMan PCR assay has been developed
and evaluated for rapid and specific detection of the brown rot pathogens M. fructicola, M. …

Isolation of laccase gene-specific sequences from white rot and brown rot fungi by PCR

TM D'Souza, K Boominathan… - Applied and …, 1996 - Am Soc Microbiol
… To rule out the possibility of interference by peroxidase, the ABTS assays were repeated
by adding excess catalase to the extracellular culture ffuids to remove H2O2. These catalase-…

Identification of root rot fungi in nursery seedlings by nested multiplex PCR

RC Hamelin, P Bérubé, M Gignac… - Applied and …, 1996 - Am Soc Microbiol
… with the PCR assay. This is important because this fungus is believed to be the primary root
rot … In addition, this fungus was detected by PCR in seedlings from which it had not been …

A multiplex PCR‐based method for the detection and early identification of wood rotting fungi in standing trees

F Guglielmo, SE Bergemann, P Gonthier… - Journal of Applied …, 2007 - academic.oup.com
… The objectives of this study were (i) to design taxon‐specific primers for wood rotting fungi
which can cause mechanical failures in urban trees; (ii) to develop multiplex PCR assays by …

A review of selected methods for the detection of degradative fungi

J Jellison, C Jasalavich - International Biodeterioration & Biodegradation, 2000 - Elsevier
… More recently we have developed a polymerase chain reaction assay which can … assays
We have also developed a DNA-based assay to reliably detect brown rot and white rot fungi in …

Qualitative and quantitative PCR methods using species-specific primer for detection and identification of wood rot fungi

S Horisawa, Y Sakuma, S Doi - Journal of Wood Science, 2009 - Springer
… The quantitative PCR assay to detect the target species from mixed-genome DNA was
examined (Fig. 4). The GtrR1 and Tve-R1 detected each target species quantitatively, and the …

Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA

CA Jasalavich, A Ostrofsky… - Applied and environmental …, 2000 - Am Soc Microbiol
… based assay to reliably detect brown rot and white rot fungi in … PCR assay conditions for
specific detection of brown-rot and white-rot fungi in wood; (iii) to identify the PCR-detected fungi

A Real-Time, Quantitative PCR Seed Assay for Botrytis spp. that Cause Neck Rot of Onion

MI Chilvers, LJ du Toit, H Akamatsu… - Plant …, 2007 - Am Phytopath Society
… protocol for assaying onion seed for neck rot fungi, which compounds the controversy …
The real-time PCR assay developed in this study for detection of neck rot Botrytis spp. has …

Species‐specific PCR Assays for the Fungal Pathogens Fusarium moniliforme and Fusarium subglutinans and their Application to Diagnose Maize Ear Rot Disease

EM Möller, J Chełkowski… - Journal of …, 1999 - Wiley Online Library
… The aim of this study was to develop speci_c PCR assays for F[ moniliforme and F[ sub`lutinans
which allow a rapid\ DNA!based detection and identi_cation of the two pathogens within …

A molecular diagnostic assay for the detection and identification of wood decay fungi of conifers

P Gonthier, F Guglielmo, F Sillo, L Giordano… - Forest …, 2015 - Wiley Online Library
fungi of conifers from the northern hemisphere, including serious root and butt rot agents and
comprising both white rot and brown rot fungi. … Green Real-Time PCR assays. Validation of …