A real‐time (TaqMan) PCR assay to differentiate Monilinia fructicola from other brown rot fungi of fruit crops
IR Van Brouwershaven, ML Bruil… - Plant …, 2010 - Wiley Online Library
… In this study an automated duplex real-time TaqMan PCR assay has been developed
and evaluated for rapid and specific detection of the brown rot pathogens M. fructicola, M. …
and evaluated for rapid and specific detection of the brown rot pathogens M. fructicola, M. …
Isolation of laccase gene-specific sequences from white rot and brown rot fungi by PCR
TM D'Souza, K Boominathan… - Applied and …, 1996 - Am Soc Microbiol
… To rule out the possibility of interference by peroxidase, the ABTS assays were repeated
by adding excess catalase to the extracellular culture ffuids to remove H2O2. These catalase-…
by adding excess catalase to the extracellular culture ffuids to remove H2O2. These catalase-…
Identification of root rot fungi in nursery seedlings by nested multiplex PCR
RC Hamelin, P Bérubé, M Gignac… - Applied and …, 1996 - Am Soc Microbiol
… with the PCR assay. This is important because this fungus is believed to be the primary root
rot … In addition, this fungus was detected by PCR in seedlings from which it had not been …
rot … In addition, this fungus was detected by PCR in seedlings from which it had not been …
A multiplex PCR‐based method for the detection and early identification of wood rotting fungi in standing trees
F Guglielmo, SE Bergemann, P Gonthier… - Journal of Applied …, 2007 - academic.oup.com
… The objectives of this study were (i) to design taxon‐specific primers for wood rotting fungi
which can cause mechanical failures in urban trees; (ii) to develop multiplex PCR assays by …
which can cause mechanical failures in urban trees; (ii) to develop multiplex PCR assays by …
A review of selected methods for the detection of degradative fungi
J Jellison, C Jasalavich - International Biodeterioration & Biodegradation, 2000 - Elsevier
… More recently we have developed a polymerase chain reaction assay which can … assays
We have also developed a DNA-based assay to reliably detect brown rot and white rot fungi in …
We have also developed a DNA-based assay to reliably detect brown rot and white rot fungi in …
Qualitative and quantitative PCR methods using species-specific primer for detection and identification of wood rot fungi
S Horisawa, Y Sakuma, S Doi - Journal of Wood Science, 2009 - Springer
… The quantitative PCR assay to detect the target species from mixed-genome DNA was
examined (Fig. 4). The GtrR1 and Tve-R1 detected each target species quantitatively, and the …
examined (Fig. 4). The GtrR1 and Tve-R1 detected each target species quantitatively, and the …
Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA
CA Jasalavich, A Ostrofsky… - Applied and environmental …, 2000 - Am Soc Microbiol
… based assay to reliably detect brown rot and white rot fungi in … PCR assay conditions for
specific detection of brown-rot and white-rot fungi in wood; (iii) to identify the PCR-detected fungi …
specific detection of brown-rot and white-rot fungi in wood; (iii) to identify the PCR-detected fungi …
A Real-Time, Quantitative PCR Seed Assay for Botrytis spp. that Cause Neck Rot of Onion
MI Chilvers, LJ du Toit, H Akamatsu… - Plant …, 2007 - Am Phytopath Society
… protocol for assaying onion seed for neck rot fungi, which compounds the controversy …
The real-time PCR assay developed in this study for detection of neck rot Botrytis spp. has …
The real-time PCR assay developed in this study for detection of neck rot Botrytis spp. has …
Species‐specific PCR Assays for the Fungal Pathogens Fusarium moniliforme and Fusarium subglutinans and their Application to Diagnose Maize Ear Rot Disease
EM Möller, J Chełkowski… - Journal of …, 1999 - Wiley Online Library
… The aim of this study was to develop speci_c PCR assays for F[ moniliforme and F[ sub`lutinans
which allow a rapid\ DNA!based detection and identi_cation of the two pathogens within …
which allow a rapid\ DNA!based detection and identi_cation of the two pathogens within …
A molecular diagnostic assay for the detection and identification of wood decay fungi of conifers
P Gonthier, F Guglielmo, F Sillo, L Giordano… - Forest …, 2015 - Wiley Online Library
… fungi of conifers from the northern hemisphere, including serious root and butt rot agents and
comprising both white rot and brown rot fungi. … Green Real-Time PCR assays. Validation of …
comprising both white rot and brown rot fungi. … Green Real-Time PCR assays. Validation of …
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