Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR
… the developed CTC gene expression RT-qPCR assay by using … For each gene target a
calibration curve was generated … efficiency expressed as E = [10 -1/slope ] -1 for each gene target …
calibration curve was generated … efficiency expressed as E = [10 -1/slope ] -1 for each gene target …
How to do successful gene expression analysis using real-time PCR
S Derveaux, J Vandesompele, J Hellemans - Methods, 2010 - Elsevier
… Reverse transcription quantitative PCR (RT-qPCR) is … sensitive and fast measurement of
gene expression. Unfortunately, what … gene expression profiling using the RT-qPCR technology. …
gene expression. Unfortunately, what … gene expression profiling using the RT-qPCR technology. …
Bacterial reference genes for gene expression studies by RT-qPCR: survey and analysis
DJP Rocha, CS Santos, LGC Pacheco - Antonie Van Leeuwenhoek, 2015 - Springer
… genes that have been most commonly selected and validated as reference genes in gene
expression studies by RT-qPCR, … We were able to compile a list of genes that might serve as a …
expression studies by RT-qPCR, … We were able to compile a list of genes that might serve as a …
Monitoring gene expression: quantitative real-time rt-PCR
EM Wagner - … and Cardiovascular Disease: Methods and Protocols, 2013 - Springer
… This chapter describes the entire procedure from isolation of total RNA from liver and fatty
tissues/cells to the use of RT-qPCR to study gene expression in these tissues. We perform …
tissues/cells to the use of RT-qPCR to study gene expression in these tissues. We perform …
Identification of valid reference genes for the normalization of RT qPCR gene expression data in human brain tissue
DTR Coulson, S Brockbank, JG Quinn, S Murphy… - BMC molecular …, 2008 - Springer
… genes are generally used to normalize RT qPCR data. Given that expression of some commonly
used reference genes is … establish which reference genes were stably expressed in post …
used reference genes is … establish which reference genes were stably expressed in post …
An overview of real-time quantitative PCR: applications to quantify cytokine gene expression
A Giulietti, L Overbergh, D Valckx, B Decallonne… - Methods, 2001 - Elsevier
… the analysis of cytokines expressed at very low levels. Once all parameters are optimized
and all factors Real-time RT-PCR as described in this review is a revolutionary technique in …
and all factors Real-time RT-PCR as described in this review is a revolutionary technique in …
Selection of reference genes for the normalization of RT-qPCR data in gene expression studies in insects: a systematic review
J Lü, C Yang, Y Zhang, H Pan - Frontiers in physiology, 2018 - frontiersin.org
… for reliable measurements of gene expression levels. Here, by … gene selection for insect
gene expression studies that employed the most widely used SYBR Green method for RT-qPCR …
gene expression studies that employed the most widely used SYBR Green method for RT-qPCR …
Twenty-five years of quantitative PCR for gene expression analysis
HD VanGuilder, KE Vrana, WM Freeman - Biotechniques, 2008 - Taylor & Francis
… expression of genes of interest, validating microarray experiments, and monitoring biomarkers.
The use of real-time qPCR … current state of qPCR for gene expression analysis now that …
The use of real-time qPCR … current state of qPCR for gene expression analysis now that …
Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method
KJ Livak, TD Schmittgen - methods, 2001 - Elsevier
… The 2 ΔΔCT method may be ase chain reaction (RT-PCR) has proven … gene expression
ful method to quantify gene expression (1–3). Real- determined from real-time quantitative PCR …
ful method to quantify gene expression (1–3). Real- determined from real-time quantitative PCR …
Accurate RT-qPCR gene expression analysis on cell culture lysates
G Van Peer, P Mestdagh, J Vandesompele - Scientific reports, 2012 - nature.com
… In conclusion, we here provide solid evidence for the accuracy of RT-qPCR gene
expression quantification using cell lysates as an input for reverse transcription, along with a …
expression quantification using cell lysates as an input for reverse transcription, along with a …