… Escherichia coli as recombinant proteins containing a C-terminal hexahistidine tag and
purified by nickel affinity chromatography. The purity of the recombinant allergens was analyzed …

… Finally, we performed MS analysis of the purified recombinant protein (fig. 1c). The molecular
… First, we tested the natural and the recombinant proteins by ELISA. A good correlation was …

… The Salsola kali pollen is considered the main cause of … recombinant Lactococcus lactis
producing Sal k1 protein with … The PNZ8148Sal k1 recombinant plasmid was transformed into …

… -binding protein band of 28 kDa is observed when Salsola kali pollen … kali pollen extract, we
identified this protein band as an … ability of the recombinant protein to activate basophils of S. …

… recombinant proteins. During the last decade, the production of recombinant allergens has
in turn allowed scientists to overcome many limitations of cross-reactivity studies and epitope …

… mice, which inhibited the binding of patients IgE to recombinant proteins. The result showed
that the designed vaccine, unlike the recombinant protein and extract, did not induce T-cell …

… The recombinant protein was correctly processed and its structural and immunologic
equivalence to the natural form was proven. A sensitization frequency between 30 and 40% was …

… Analysis of purified recombinant proteins revealed that the recombinant forms of both
fragments of the Sal k 3 were reactive with eight individual sera (Fig. 1c and d, lane 1, 2, 3, 5, 6, 9, …

… kali pollen extract and purified recombinant protein revealed that they were reactive with 14
(46.6%) individual sera (Fig. 3B and C), lanes 1, 2, 3, 6, 9, 11, 13, 14, 16, 17, 20, 24, 27, and …

… Abstract Objective: The Salsola kali pollen is considered as a … recombinant Lactococcus
lactis producing Sal k1 protein with … The PNZ8148-Sal k1 recombinant plasmid was transformed …