Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR

M Park, J Won, BY Choi, CJ Lee - Experimental & molecular medicine, 2020 - nature.com
… The three steps include (1) the selection of primer sets for target genes (RdRP, N, E, and
S) in the genome of interest (SARS-CoV-2), (2) the in silico validation of primer and amplicon …

Antiviral activity of chitosan nanoparticles encapsulating silymarin (Sil–CNPs) against SARS-CoV-2 (in silico and in vitro study)

SA Loutfy, AI Abdel-Salam, Y Moatasim, MR Gomaa… - RSC …, 2022 - pubs.rsc.org
… alone to prevent viral infectivity, as we still could detect viral copies/mL using real-time RT
PCR … In addition, our results were supported by in silico analysis which showed high binding …

In silico evaluation of the impact of Omicron variant of concern sublineage BA. 4 and BA. 5 on the sensitivity of RT‐qPCR assays for SARSCoV‐2 detection using …

D Sharma, KI Notarte, RA Fernandez… - Journal of Medical …, 2023 - Wiley Online Library
… In this study, the in silico sensitivity of 12 RT-qPCR tests (containing 30 primers and probe
sets) developed for detection of SARS-CoV-2 was assessed for detecting SARS-CoV-2 BA.4 …

[PDF][PDF] n Silico Design of Specific Primer Sets for the Detection of B. 1.1. 529 SARS-CoV-2 Variant of Concern(Omicron)

M Petrillo, M Querci, P Corbisier, A Marchini… - Zenodo, 2021 - academia.edu
silico developed RT-PCR based detection method (named OmMet) that was designed to be
highly specific for the detection … , including other coronaviruses or SARS-CoV-2 variants. The …

In silico comparative genomics of SARS-CoV-2 to determine the source and diversity of the pathogen in Bangladesh

TA Shishir, IB Naser, SM Faruque - PLoS One, 2021 - journals.plos.org
… severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), … , namely SARS-CoV and
MERS-CoV demonstrated high … We detected the presence of 209 point mutations in 64 SARS

A new advanced in silico drug discovery method for novel coronavirus (SARS-CoV-2) with tensor decomposition-based unsupervised feature extraction

YH Taguchi, T Turki - PloS one, 2020 - journals.plos.org
… Thus, the PPI detected in this study (S1 Table) is not accidental but reliable. … this is the first
report of an in silico approach that can detect ivermectin as a possible SARS-CoV-2 drug. This …

[HTML][HTML] In silico evaluation of the impact of the Omicron variant on the sensitivity of RT-qPCR assays for SARS-CoV-2 detection using whole genome sequencing

D Sharma, C Ye, G Lippi, JB Torrelles… - 2022 - europepmc.org
… In this study, the in silico sensitivity of 12 RT-qPCR tests (containing 30 primers and probe
sets) developed for detection of SARS-CoV-2 was assessed for use in detecting SARS-CoV-2 …

[HTML][HTML] Detection of SARS-CoV-2 variants by Abbott molecular, antigen, and serological tests

MA Rodgers, A Olivo, BJ Harris, C Lark, X Luo… - Journal of Clinical …, 2022 - Elsevier
… In silico analysis of 746,345 sequences from 45 SARS-CoV-2 lineages revealed no
significant mutations of concern for the performance of Abbott assays targeting the RNA-dependent …

Performance evaluation of the SAMBA II SARS-CoV-2 test for point-of-care detection of SARS-CoV-2

SM Assennato, AV Ritchie, C Nadala… - Journal of Clinical …, 2020 - Am Soc Microbiol
… for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in
… (SAMBA) II SARS-CoV-2 test were determined by both in silico analyses of the primers and …

Identification of Unique Peptides for SARS-CoV-2 Diagnostics and Vaccine Development by an In Silico Proteomics Approach

V Kesarwani, R Gupta, RR Vetukuri… - Frontiers in …, 2021 - frontiersin.org
… have detected polypeptides of the SARS-CoV-2 nucleoprotein (20, 21), and several in-silico
efforts have been made to identify antigenic peptides, T- and B-cell epitopes of SARS-CoV-…