Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion (Neophoca cinerea) and other mammals
MIM Cerda, R Gray, DP Higgins - PeerJ, 2020 - peerj.com
… This study describes the development and validation of cross-reactive RT-qPCR assays to …
species including the Australian sea lion. All RT-qPCR assays efficiencies ranged between 87…
species including the Australian sea lion. All RT-qPCR assays efficiencies ranged between 87…
Development of a real-time quantitative RT-PCR (Taqman®) assay to measure cytokine profiles in California sea lions (Zalophus californianus) and Bottlenose …
C Funke, A Aldridge, C Leutenegger, BR Smith… - … Association for Aquatic …, 2002 - vin.com
… and IL-2, IL-10, and IL12p40 genes in the California sea lion. In both species real time
quantitative PCR was successfully performed for each of these cytokines (Figures 1 and 2) except …
quantitative PCR was successfully performed for each of these cytokines (Figures 1 and 2) except …
A nutrigenomic approach to detect nutritional stress from gene expression in blood samples drawn from Steller sea lions
… stress in Steller sea lions (Eumetopias jubatus). We subjected 4 female Steller sea lions to
3 feeding … Specific primers used in quantitative PCR analysis designed from Steller sea lion …
3 feeding … Specific primers used in quantitative PCR analysis designed from Steller sea lion …
[PDF][PDF] Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion
MIM Cerda, R Gray, DP Higgins - academia.edu
… This study describes the development and validation of cross-reactive RT-qPCR assays to …
species including the Australian sea lion. All RT-qPCR assays efficiencies ranged between 87…
species including the Australian sea lion. All RT-qPCR assays efficiencies ranged between 87…
Development and validation of a quantitative PCR for rapid and specific detection of California sea lion adenovirus 1 and prevalence in wild and managed …
G Cortés-Hinojosa, FMD Gulland… - Journal of …, 2017 - journals.sagepub.com
… California sea lion adenovirus 1 (CSLAdV-1) has been associated with hepatitis and … PCR
(qPCR) assay for rapid, sensitive, and specific detection of this virus in California sea lions (…
(qPCR) assay for rapid, sensitive, and specific detection of this virus in California sea lions (…
Unlocking the role of a genital herpesvirus, otarine herpesvirus 1, in california sea lion cervical cancer
AC Deming, JFX Wellehan, KM Colegrove, A Hall… - Animals, 2021 - mdpi.com
… , quantified viral load from cervical tissue from sea lions with (n = 95) and … sea lions diagnosed
with urogenital carcinoma, 100% were qPCR positive for OtHV1, and 36% of the sea lions …
with urogenital carcinoma, 100% were qPCR positive for OtHV1, and 36% of the sea lions …
PCR‐based techniques to determine diet of the Australian sea lion (Neophoca cinerea): a comparison with morphological analysis
KJ Peters, K Ophelkeller, Herdina, NJ Bott… - Marine …, 2015 - Wiley Online Library
… quantitative real-time PCR (qPCR) DNA-based techniques to determine their suitability to
amplify and detect fish, shark and cephalopod prey DNA recovered from Australian sea lion …
amplify and detect fish, shark and cephalopod prey DNA recovered from Australian sea lion …
Differential Expression of Immune Response Genes in Steller Sea Lions (Eumetopias jubatus): An Indicator of Ecosystem Health?
L Bowen, B Aldridge, K Beckmen, T Gelatt, L Rea… - EcoHealth, 2006 - Springer
… In light of this concept, we developed a quantitative PCR system to examine MHC class II
DRB gene expression in Alaskan Steller sea lions (SSL; Eumetopias jubatus) from two …
DRB gene expression in Alaskan Steller sea lions (SSL; Eumetopias jubatus) from two …
Health status, infection and disease in California sea lions (Zalophus californianus) studied using a canine microarray platform and machine-learning approaches
A Mancia, JC Ryan, RW Chapman, Q Wu… - Developmental & …, 2012 - Elsevier
… We used the microarray to interrogate California sea lion health and diseases. ► Resulting
… Relative levels of mRNA were determined using qPCR on an ABI7500 (Applied Biosystems, …
… Relative levels of mRNA were determined using qPCR on an ABI7500 (Applied Biosystems, …
Comparison of paired sex ratio estimates obtained from novel molecular assays and visual surveys in the sexually dimorphic Steller sea lion (Eumetopias jubatus)
M Gard, ZK Lewis, AM Akmajian… - Conservation Genetics …, 2024 - Springer
… Here, we describe a real-time qPCR method specifically designed to determine the sex of
… sea lion and the California sea lion— from scat. Specifically, we developed Taqman qPCR …
… sea lion and the California sea lion— from scat. Specifically, we developed Taqman qPCR …
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