… for the RNA isolation step and RT-PCR. By using limited primer … real-time RT-PCR proved
to have a sensitivity of approximately eight copies. The primer–probe combination selected …

… In order to validate the use of the genes selected by geNorm and NormFinder algorithms
as reference genes, we examine the effect of using different HKGs on the qRT-PCR-determined …

… analysis by reverse transcriptase real-time or quantitative polymerase chain reaction (RT-…
or reference genes is critical, and careful selection of these reference genes is one of the most …

… The expressions of selected reference genes, which were limited to the traditional ones …
In the present study, the objective was to select superior reference genes for reliable qRT-PCR …

For reliable results, Reverse Transcription Quantitative real-time Polymerase Chain Reaction
(RT-qPCR) analyses depend on stably expressed reference genes for data normalization …

… be validated in the system of interest prior to its use in RT-PCR. This strategy is being
recommended by more and more researchers (Aerts et al, 2004; Dheda et al, 2004) using RT-PCR …

… Our selection of subjects and time points mirrored experimental protocols that we propose
… We selected group 2 genes to increase the number of genes considered in our validation …

… The assessed lack of validated reference genes emphasizes the importance of a systematic
study for their identification. For selecting candidate reference genes we have developed a …

… selected based on the whole genome sequences of V. angularis and among which the most
stable reference genes were validated … , we can also select stable reference genes from …

… had been validated as references were used in only 3.2% of 188 real-time RT-PCR analyses
… Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic …