Detecting SARS-CoV-2 variants with SNP genotyping

H Harper, A Burridge, M Winfield, A Finn, A Davidson… - PloS one, 2021 - journals.plos.org
… -RT genotyping assays could provide useful viral genotype identification for UK SARS-CoV-2 …
markers; initial evaluation of a set of two SARS-CoV-2 isolates (GBR/Liverpool_strain/2020 …

[HTML][HTML] Detection of SARS-CoV-2 VOC-Omicron using commercial sample-to-answer real-time RT-PCR platforms and melting curve-based SNP assays

BHM Sit, KHL Po, YY Cheung, AKL Tsang… - Journal of Clinical …, 2022 - Elsevier
… In our study, we assessed four commercially available SARS-CoV-2 Spike SNP assays,
which can be easily adapted in routine screening for Omicron variants. The signature mutations …

[HTML][HTML] Monitoring the SARS-CoV-2 pandemic: screening algorithm with single nucleotide polymorphism detection for the rapid identification of established and …

J Mertens, J Coppens, K Loens, M Le Mercier… - Clinical Microbiology …, 2022 - Elsevier
… acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants that includes the use of
PCR-based targeted single nucleotide polymorphism (SNP) detection assays preceded by a …

Rapid automated screening for SARS-CoV-2 B. 1.617 lineage variants (Delta/Kappa) through a versatile toolset of QPCR-based SNP detection

D Nörz, M Grunwald, HT Tang, F Olearo, T Günther… - Diagnostics, 2021 - mdpi.com
SARS-CoV-2 … SNP detection, which was applied to screen for SARS-CoV-2 B.1.617
lineage variants. Methods: We created a broad set of single nucleotide polymorphism (SNP)-assays

A novel single nucleotide polymorphism assay for the detection of N501Y SARS-CoV-2 variants

MS Torrientes, CC Abietar, JB Riveiro… - Journal of Virological …, 2021 - Elsevier
… The N501Y mutation in SARS-CoV-2 variants found in several strains from the UK, South …
, a single nucleotide polymorphism (SNP) discrimination assay was designed and validated. It …

Evaluation of a fully automated high-throughput SARS-CoV-2 multiplex qPCR assay with built-in screening functionality for del-HV69/70-and N501Y variants such as …

D Nörz, M Grunwald, F Olearo, N Fischer… - Journal of Clinical …, 2021 - Elsevier
… of an N501Y SNP was 105.99 cp/ml (CI: 81.59 – 183.66), indicating that the N501Y SNP-assay
is less sensitive than the two diagnostic-grade SARS-CoV-2 assays. All detection limits …

SNP and phylogenetic characterization of low viral load SARS-CoV-2 specimens by target enrichment

GS Orf, K Forberg, TV Meyer, I Mowerman… - Frontiers in …, 2021 - frontiersin.org
… specimens to the Abbott RealTime SARS-CoV-2 qPCR EUA assay. This assay has 10 “dark” …
our laboratory by the Abbott RealTime SARS-CoV-2 qPCR assay, even though they tested …

SARS-CoV-2 variant determination through SNP assays in samples from industry workers from Rio de Janeiro, Brazil

BM Henriques-Santos, B Farjun, IA Corrêa… - Frontiers in …, 2022 - frontiersin.org
Since the first reported case in December 2019, SARS-CoV-… due to the emergence of new
SARS-CoV-2 variants. Despite … circulation of currently identified SARS-CoV-2 variants within …

SARS-CoV-2 variants detection using TaqMan SARS-CoV-2 mutation panel molecular genotyping assays

P Neopane, J Nypaver, R Shrestha… - Infection and Drug …, 2021 - Taylor & Francis
SARS-CoV-2 mutation panel genotyping molecular assay for detection of most common
reported SARS-CoV-2 variants using specific RT-PCR assays … TaqMan SNP genotyping assays

Detection of SARS-CoV-2 variants via different diagnostics assays based on single-nucleotide polymorphism analysis

E Specchiarello, G Matusali, F Carletti, CEM Gruber… - Diagnostics, 2023 - mdpi.com
… Although full-genome sequencing remains the elected method to characterize new strains,
our data show the high ability of a SNP-based assay to identify VOCs, also resolving samples …