… protein fragments for soluble expression in Escherichia coli from a randomly fragmented
gene … Bacterial strains expressing mDHFR fusions with the soluble proteins green fluroscent …

… solubility to largely facilitate the folding of the fusion partner (Pédelacq et al. 2006). In this
study, we obtained soluble expression … sfGFP-scFv-His remained soluble in the cell cytoplasm (…

… To achieve soluble expression and convenient purification of the target scFvs, the strategy
using dual tag sequences, the solubility-enhancing and affinity tag sequences, has been …

… Here, we report up to 14-fold increase in the soluble expression of the scFv primarily as a
result of codon optimization with minor effects from inter-domain peptide linkers and variable …

… In this study, however, coexpression of DnaK-DnaJ-GrpE was sufficient to enhance soluble
expression of the target scFv, ie, coexpression of GroEL-GroES-DnaK-DnaJ-GrpE was not as …

… of variable domain orientation on soluble expression yield in … Hence, soluble expression
yield and bioactivity of scFv-… make an important addition about soluble expression and binding …

… line producing MC3 and the DNAs encoding variable domains of heavy and light chains (…
AGS highly expressing MC3-binding antigen, the phage clones displaying ScFv fragments of …

… of the variable domains of these two antibodies in comparison with those antibodies
whose scFv were only produced in a soluble state when CyDisCo components were present. …

… Here, we described that co-expression of appropriate disulfide … the soluble expression
of target scFv in E. coli. A 50 % recovery in solubility of the scFv was observed upon co-expression …

… of recombinant antibody fragment molecules, … soluble expression was ensured and soluble
scFv was liberated in cells via autonomous proteolytic cleavage by endogenously expressed …