Sperm selection using magnetic activated cell sorting (MACS) in assisted reproduction: a systematic review and meta-analysis

M Gil, V Sar-Shalom, Y Melendez Sivira… - Journal of assisted …, 2013 - Springer
… To determine whether the use of Magnetic Activated Cell Sorting (MACS) as a sperm
selection technique improves ART success rates in couples undergoing assisted reproduction …

[HTML][HTML] Magnetic-activated cell sorting (MACS): a useful sperm-selection technique in cases of high levels of sperm DNA fragmentation

A Pacheco, A Blanco, F Bronet, M Cruz… - Journal of Clinical …, 2020 - mdpi.com
… Magnetic-activated cell sorting (MACS) can be used to separate apoptotic sperm with high
… high levels of sperm DNA fragmentation (SDF) undergoing intracytoplasmic sperm-injection (…

Sperm selection methods in the 21st century

DA Vaughan, D Sakkas - Biology of reproduction, 2019 - academic.oup.com
… The first uses magnetic activated cell sorting (MACS) with colloidal superparamagnetic
microbeads conjugated with annexin V. The sample is passed through a column containing …

Does combining magnetic-activated cell sorting with density gradient or swim-up improve sperm selection?

Z Cakar, B Cetinkaya, D Aras, B Koca… - Journal of assisted …, 2016 - Springer
sperm injection (ICSI) cycles. These techniques can be classified in a wide range … sperm
washing to advanced sperm selection methods including the removal of apoptotic cells, sperm

[HTML][HTML] Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter

S Nakao, T Takeo, H Watanabe, G Kondoh… - Scientific reports, 2020 - nature.com
cells 10 . Therefore, we considered that the microfluidics chip cell sorter may be useful to
select fertile sperm… the microfluidics chip cell sorter is useful for sperm selection by using the …

Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities

G Marzano, MS Chiriacò, E Primiceri… - Biotechnology …, 2020 - Elsevier
sperm selection compared to conventional sperm sorting techniques by isolating mature,
membrane and chromatin-intact sperm… to be overcome, such as sperm damage, low yield, high …

Sex preselection: high-speed flow cytometric sorting of X and Y sperm for maximum efficiency

LA Johnson, GR Welch - Theriogenology, 1999 - Elsevier
… We acquired a cell sorter in 1982 and modified it to sort sperm nuclei (17). This system
formed the basis for the current technology as various protocols were developed that led to …

Chromosomal scan of single sperm cells by combining fluorescence-activated cell sorting and next-generation sequencing

QT Tran, T Jatsenko, O Poolamets, O Tšuiko… - Journal of assisted …, 2019 - Springer
… In this study, we optimized a FC-based protocol for single sperm cell sorting and lysis, and
sperm cells, we optimized the cell sorting conditions and used fluorescent dye to label cells. …

Density gradient centrifugation before or after magnetic-activated cell sorting: which technique is more useful for clinical sperm selection?

M Tavalaee, MR Deemeh, M Arbabian… - Journal of Assisted …, 2012 - Springer
… The results of this study show that for sperm preparation or sperm selection, magnateic-activated
cell sorting (MACS) should be carried out before Density gradient centrifugation (DGC) …

Sex-sorting sperm using flow cytometry/cell sorting

DL Garner, KM Evans, GE Seidel - Spermatogenesis: Methods and …, 2013 - Springer
… development of a flow sorting system called a fluorescence-activated cell sorter (FACS) that
… first actual sorting of mammalian sperm into separate populations was achieved with sperm