… Human adipose-derived stromal cell spheroids also showed … Our results suggest that spheroid
culture augmented the … 3D spheroid culture improved the secretion of angiogenic factors, …

… changes resulting from different NOS-3 expression levels and activities (26). As 3-dimensional
spheroid cell culture conditions have been shown to overcome some of these limitations, …

… As such, the spheroid culture model lends itself to a plethora of experimental approaches:
(a) The analysis of the angiogenic potential and responses of vascular ECs, mimicking …

… -dimensional (2D) culture dishes. Although these assay … of angiogenesis modulators, 2D
culture systems have some limitations and drawbacks. One of the major limitations of 2D culture …

… spheroids have been established during the last years. Due to their unique angiogenic and
vasculogenic properties, spheroids … spheroid cultures. Thereby, it should be considered that …

… After optimizing the cell seeding density, we investigated the effect of culture time on the
compaction, angiogenic paracrine factor expression, and cell–cell interactions in …

… angiogenesis in a 3D environment. Endothelial cells are cultured as hanging drops to form
spheroids. … Use supernatant of the methocel solution for spheroid culture 2. Collagen stock …

… of heterotypic co-culture spheroids in angiogenic assays. Although the EC-… spheroids was
higher than that in the EC-only spheroids and correlated with in vivo antiangiogenic activity of …

… We report a spheroid-based stem cell therapy for postnatal angiogenesis in ischemic tissue.
Three-dimensional spheroid cultures of hADSCs significantly improved cell survival under …

… Endothelial cell (EC) spheroids were prepared by pipetting 500 EC in a hanging drop (25
μl) on plastic dishes to allow overnight spheroid aggregation under culture conditions of 37 C, …