Spheroid culture of mesenchymal stem cells

Z Cesarz, K Tamama - Stem cells international, 2016 - Wiley Online Library
… Cytoskeletal reorganization and drastic changes in cell morphology in MSC spheroids
enhanced stemness in MSC spheroids. Furthermore, spheroid formation causes drastic changes …

Spheroid-formation (colonosphere) assay for in vitro assessment and expansion of stem cells in colon cancer

S Shaheen, M Ahmed, F Lorenzi, AS Nateri - Stem cell reviews and reports, 2016 - Springer
… which may influence the formation efficiency and other … established spheroid formation from
human colon cancer cells using … cell lines but no significant difference in spheroid formation

Spheroid culture system methods and applications for mesenchymal stem cells

NE Ryu, SH Lee, H Park - Cells, 2019 - mdpi.com
… In particular, cadherin and integrin are directly related to the mechanism of spheroid
formation. The process of spheroid formation is divided into several steps. Firstly, single cells

The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities

NC Cheng, S Wang, TH Young - Biomaterials, 2012 - Elsevier
… To prevent necrosis of the spheroid core, we opted for spheroids formed with a seeding
density of 2.50 × 10 … cells/cm 2 in the following experiments. The ASC aggregates grown at this …

Short-term spheroid formation enhances the regenerative capacity of adipose-derived stem cells by promoting stemness, angiogenesis, and chemotaxis

NC Cheng, SY Chen, JR Li… - Stem Cells Translational …, 2013 - academic.oup.com
… by further cell expansion in monolayers. … -term spheroid formation to increase the regenerative
capabilities of ASCs for clinical application. Our results show that ASCs within spheroids

The effects of spheroid formation of adipose-derived stem cells in a microgravity bioreactor on stemness properties and therapeutic potential

S Zhang, P Liu, L Chen, Y Wang, Z Wang, B Zhang - Biomaterials, 2015 - Elsevier
… acute liver failure, spheroid-derived ADSCs … spheroid formation of ADSCs in an MB
enhances their stemness properties and increases their therapeutic potential. Therefore, spheroid

Spheroid formation of mesenchymal stem cells on chitosan and chitosan-hyaluronan membranes

GS Huang, LG Dai, BL Yen, S Hsu - Biomaterials, 2011 - Elsevier
Spheroid formation had a significant impact on the stemness marker gene expression of these
stem cells… (ROCK) on the spheroid formation was investigated. Possible mechanisms for …

Spheroid formation and differentiation into hepatocyte-like cells of rat mesenchymal stem cell induced by co-culture with liver cells

Z Qihao, C Xigu, C Guanghui, Z Weiwei - DNA and cell biology, 2007 - liebertpub.com
… Mesenchymal stem cells (MSCs) co-cultured with liver cells produced and stored glycogen
… Glycogen was also present in spheroids formed by sorted MSCs after a 21-day liver cell co-…

Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells

G Oktem, A Bilir, R Uslu, SV Inan… - Oncology …, 2014 - spandidos-publications.com
stem cell markers increased following the differentiation of CSCs when the cells constituted a
spheroid formation. … whether the cells were maintained in monolayer culture or as spheroid. …

Hypoxic preconditioning of mesenchymal stem cells with subsequent spheroid formation accelerates repair of segmental bone defects

SS Ho, BP Hung, N Heyrani, MA Lee, JK Leach - Stem Cells, 2018 - academic.oup.com
formation in vivo. We hypothesized that preconditioning MSCs in hypoxic culture before
spheroid formation would increase cellcell viability of spheroids formed from increasing cell