… WT Spike or D614G Spike, titered and normalized by the level of HIV p24 Gag protein and
used to infect control 293T, and 293T cells stably expressing mouse ACE2 or human ACE2. …

… given level of cell-surface ACE2 or … level of ACE2 together with a range of expression levels
of TMPRSS2 and vice versa (Fig. 1b, c), SARS2-S-mediated infection required higher levels …

… To rapidly assess mutations in a soluble format, we fused the ACE2 protease domain to
sfGFP. Expression levels of sACE2-sfGFP were evaluated qualitatively by fluorescence (fig. S4A)…

… Further studies using reverse genetics are required to confirm the relevance of our findings,
connecting the D614G mutation with different ACE2 expression levels. Other factors, such as …

… previous in silico analysis study (17); similarly, our pseudotyped virus entry assay showed
that mink ACE2 allowed viral entry, although at a relatively lower level than that observed with …

… We hypothesized that the high levels of ACE2 expression from the Tet-inducible promoter
… loss of affinity from ACE2 mutation. To test this, we reduced the amount of ACE2 cell surface …

… The k off (B) and k on (C) values measured at different levels of immobilised ACE2 are shown.
(D) … (E) The equilibrium K D measured at different levels of immobilised ACE2 are shown. …

… of ACE2 and their effect on expression levels of ACE2 are also provided in this review. Such
studies can improve the prediction of the affinity of mutant ACE2 variations with spike protein…

… -mutation variants (V367F+D614G), which may derivate from recombination. The analysis of
critical RBD mutations … supports the continuing surveillance of spike mutations to aid in the …

… ACE2, 14 and in vitro mutation analysis of human ACE2 15 … the RBD of viral spike protein
and ACE2 peptidase domain. In … ) alleles of ACE2 may offer some level of resistance against …