… Gene circuits will not function if a constituent gene is not expressed, or if a regulator lacks …
any synthetic gene circuit from microbes into mammalian cells. Even for gene circuits with …

… of new strategies to treat human diseases (Fig. 1). This review highlights the latest state-of-the-art
synthetic gene circuits and prototype treatment strategies for therapeutic purposes. …

… mammalian cells. We cover classic and emerging design strategies for synthetic gene …
for connecting basic gene switches to build more complex gene circuits with preset network …

… The design and construction of synthetic gene circuits with complex … synthetic biologists
were able to construct genetic circuits with complex spatiotemporal dynamics in mammalian cells…

… for synthetic network design is ever-advancing and has still ample room to grow. Here, we
review the diversity of synthetic gene … in the control of mammalian synthetic genetic circuits. …

… gene circuits in mammalian cells. For readers interested in the progress made in bacterial
synthetic biology, we recommend a recent review focused on engineered prokaryotic gene …

… Then, we highlight the direct impacts of synthetic gene circuits in current stem cell research,
… prospects for synthetic biology, we conclude that the scenario of using human cells as living …

… Recent studies have therefore focused on designing heterologous transcription-control
systems in mammalian cells. This research opens a door for developing new therapeutic …

… these genetic programs in mammalian cells remains a major challenge (8). Toward systematic
and rational engineering of mammalian cells… of genetic circuits in a standardized manner. …

… parameters for genetic circuits in mammalian cells, in order to enable the cells to sense a …
Challenges remain in engineering of cells for therapeutic purposes, but the field of synthetic …