Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system

X Chen, X Lu, N Shu, S Wang, J Wang, D Wang… - Scientific reports, 2017 - nature.com
… /Cas9-mediated targeted mutagenesis in cotton. Here, we report the genome editing and
targeted mutagenesis in upland cotton (Gossypium hirsutum L., hereafter cotton) using the …

A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)

C Li, T Unver, B Zhang - Scientific reports, 2017 - nature.com
… raimondii (DD; 2n = 26) 25,26 , A-genome diploids Gossypium arboreum (AA; 2n = 26) 27 ,
ant the AtDt allotetraploid cotton species Gossypium hirsutum and Gossypium barbadense 28…

CRISPR/Cas9-mediated targeted mutagenesis in upland cotton (Gossypium hirsutum L.)

MR Janga, LAM Campbell, KS Rathore - Plant molecular biology, 2017 - Springer
… system to target a gene within the genome of cotton, we made use of a transgenic cotton line
… of this powerful new tool in targeted knockout of a gene residing in the cotton genome. By …

Rich variant phenotype of Gossypium hirsutum L. saturated mutant library provides resources for cotton functional genomics and breeding

Y Wei, Y Liu, AM Ali, R Xiao, C Liang, Z Meng… - Industrial Crops and …, 2022 - Elsevier
… In this study, we obtained an upland cotton library through EMS induced mutagenesis,
further phenotypic investigation of the mutant library and evaluation of mutation efficiency were …

Mutagenesis for targeted breeding in cotton

M Rahman, S Zafar, M Hussain, H Abbas… - Cotton precision …, 2021 - Springer
… In mutagenesis experiments, usually a cultivated variety is exposed to a mutagen followed …
throughout the genome, in contrast to site-directed mutagenesis approaches. In the past more …

Cotton (Gossypium hirsutum L.) Breeding Strategies

S Rauf, M Shehzad, JM Al-Khayri, HM Imran… - Advances in Plant …, 2019 - Springer
… This chapter is focused on the achievements and future prospects of cotton breeding and
related biotechnology. Traditional plant breeding has been utilized for the development of pure-…

Construction of Gossypium barbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement

MA Abid, P Wang, T Zhu, C Liang, Z Meng… - International Journal of …, 2020 - mdpi.com
… In this study, we demonstrated the potential of EMS-induced mutagenesis to create a vast
mutant library of G. barbadense and induced genetic variability can be used to identify gene-…

Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)

G Wang, Z Xu, F Wang, Y Huang, Y Xin, S Liang, B Li… - BMC biology, 2022 - Springer
… The widely cultivated cotton species upland cotton (Gossypium hirsutum) is a global cash
crop, for the production of both natural textile fiber and seed oil. Upland cotton is allotetraploid (…

High‐efficient and precise base editing of C•G to T•A in the allotetraploid cotton (Gossypium hirsutum) genome using a modified CRISPR/Cas9 system

L Qin, J Li, Q Wang, Z Xu, L Sun… - Plant biotechnology …, 2020 - Wiley Online Library
… Therefore, it is desirable to produce novel alleles and improved traits by creating targeted
point mutations. Traditional mutagenesis techniques are not targeted and require genome-…

Ethyl methanesulfonate mutant library construction in Gossypium hirsutum L. for allotetraploid functional genomics and germplasm innovation

X Lian, Y Liu, H Guo, Y Fan, J Wu, H Guo… - The Plant …, 2020 - Wiley Online Library
cotton are widely cultivated among many varieties; the major allotetraploid cotton species are
upland cotton (Gossypium hirsutum L… In mutagenesis, suitable mutagen doses are required …