… after production of the molecule and subsequent coupling to haptens (47,48), fluorochromes
(48), … We applied the hapten digoxigenin to the enzymatic labeling of oligonucleotides. This …

… Monomethyl auristatin E was used as a cytotoxic drug, digoxigenin acted as a hapten, and
the humanized anti-digoxigenin antibody served as a universal carrier of the aptamer. The …

… Tailing with terminal transferase (Boehringer): To obtain comparable results, I pgof plasmid
… in vitro with the hapten digoxigenin by tailing with terminal trans ferase. Analyt Biochem 192:…

… ol igonucleotides with terminal transferase by incorporation of a single digoxigenin labeled
… ' end with the hapten digoxigenin (DIG) by enzymatic tailing with terminal transferase and DIG…

… The novel system is based on an alternative type of DNAmodification with the cardenolide-hapten
digoxigenin, which is recognized by digoxigenin-specific polyclonal sheep antibodies …

… to the steroid hapten digoxigenin (Fig. 1; Boehringer Mannheim). The size of the hapten and
… 3’ serial addition of Dig–UTPs by terminal transferase. Dig–dUTP also lends itself readily to …

… A further analysis could include purification of the labeled probe by agarose gel
electrophoresis and recombine that with fresh terminal transferase to evaluate the conditions for …

… the probe with digoxigenin-11… digoxigenin-11-dUTP by using terminal transferase was so
far only applied in in situ hybridisation experiments. Our aim was the routine use of digoxigenin …

… modified, terminal dideoxynucleotidyl transferase incorporates … before the activation and
conjugation of the enzyme hapten. … the hapten digoxigenin by tailing with terminal transferase. …

… Taq, reverse transcriptase, and terminal transferase. Digoxigenin can therefore be introduced
into … Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG): …